Addgene

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Viral Production at Addgene

Type

Blog Post

...lentiviral vectors. This article was written by multiple Addgenies and most recently updated by Ina Ersing...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...sequences with complex secondary structure. Today, multiple companies offer long single-stranded DNA production...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors. ...
Career Insights: Technical Support Specialist

Type

Blog Post

.... In addition, it can be advantageous to speak multiple languages - that makes communication with customers...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

... so that the marker can be reused to introduce multiple genetic modifications in the same strain. MazF...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

...Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens” ...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...be on higher salaries (which have been made by multiple organizations across academia). Increased postdoc...
The Challenges of Cell Culture

Type

Blog Post

...stocks of cells. Working stocks should be frozen in multiple vials and thawed to produce a new batch once you...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...GCN4 binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag ...
Five Popular Model Organisms, Part 2

Type

Blog Post

...Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. Overall, the ability...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...influence whether cells will be co-transduced with multiple copies of both vectors. Examples of use: Halbert...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...proved challenging to study. Cells often express multiple integrins that bind the same ligand, making it...
Tips for Getting a Faculty Position

Type

Blog Post

... need a Nature/Cell/Science paper. You do need multiple first/corresponding author publications in quality...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

... alleles of a gene, or which require modifying multiple genes simultaneously in the same cell, are more...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

...Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of the National...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

... the only fruit-named aptamer, Mango, binds to multiple derivatives of thiazole orange (TO) and fluoresces...

Showing: 501 - 520 of 544 results

Results per page:

Viral Production at Addgene

Viral Vectors 101: Preparing Pooled Libraries

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Career Insights: Technical Support Specialist

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Fluorescent Proteins 101: Aptamer Fluorophores

Changing Labor Laws Bring Increased Postdoc Wages

The Challenges of Cell Culture

CRISPR 101: Epigenetics and Editing the Epigenome

Harnessing Bacterial Toxins for Allelic Exchange

Antibodies 101: Single Chain Fragment Variables (scFvs)

Five Popular Model Organisms, Part 2

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

An Integrin Antibody Toolkit from IPI

Tips for Getting a Faculty Position

Adeno-associated Viruses (AAVs) for Genome Editing

Fluorescent Proteins 101: When GFP lets you down

The Fluorescent Vegetables in Aptamer Soup

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