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Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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Blog Post

...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....
Polymerase Chain Reaction Overview and Applications

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Blog Post

...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...
Cloning Mammalian Cells with the Agarose Method

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Blog Post

...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...
Anatomy of a Plasmid Page at Addgene

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Blog Post

..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without...
Plasmids 101: Secondary Nanobody Toolbox

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Blog Post

... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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Blog Post

...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...
Tips for arabidopsis transformation

Type

Blog Post

...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...
Tips from the Repository Trenches: Using Barcodes to Track Samples

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Blog Post

...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...
Your Lentiviral Plasmid FAQs Answered

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Blog Post

...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...
Plasmids 101: Broad Host Range Plasmids

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Blog Post

...Initiation of Replication. Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc...
Fluorescent Proteins 101: Aptamer Fluorophores

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Blog Post

...-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina...
Viral Vectors 101: Pseudotyping

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Blog Post

...virus is to delete the glycoprotein required for entry into the cell (rabies dG). Because rabies dG can...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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Blog Post

... barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS. Thus, the ...
Supporting Reproducibility with a Connected ELN

Type

Blog Post

... into the desired repository. They also support entry of required metadata, with the option of adding ...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...going to graduate school, Joe taught high school chemistry for a year. The teaching/mentoring bug never went...

Showing: 481 - 500 of 520 results

Results per page:

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Polymerase Chain Reaction Overview and Applications

Cloning Mammalian Cells with the Agarose Method

Anatomy of a Plasmid Page at Addgene

Plasmids 101: Secondary Nanobody Toolbox

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Capturing a Moment with Marc Zimmer’s “The State of Science”

Tips for arabidopsis transformation

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Tips from the Repository Trenches: Using Barcodes to Track Samples

Your Lentiviral Plasmid FAQs Answered

Plasmids 101: Broad Host Range Plasmids

Fluorescent Proteins 101: Aptamer Fluorophores

Viral Vectors 101: Pseudotyping

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

New Tools Enable CRISPRa for Neuroscience Applications

Supporting Reproducibility with a Connected ELN

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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