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Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Type

Blog Post

...enough... If in the past you have tried to combine multiple terms in an Addgene plasmid search and found the...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

...targeting a specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated...
Getting to Know Expansion Microscopy

Type

Blog Post

...Introduction to Immunofluorescence Antibodies 101: Multiplex Immunofluorescence Antibodies 101: Affinity Reagents...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...
Tips for arabidopsis transformation

Type

Blog Post

...for transformants. It’s very important to select multiple T1 plants from each transformation. I like to ...
Great Results Start with Great Standard Curves

Type

Blog Post

...measurement divided by the mean of all the replicates multiplied by 100. To calculate the %CV of the standard ...
Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...certainly chapters that could warrant their own books. Multiple books have been written about CRISPR alone, and...
Lentiviral Vector Uses and Overview

Type

Blog Post

...PMCID: PMC110254. 3. In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola...
Plasmids 101: Colony PCR

Type

Blog Post

... mutations in your insert. Make sure to submit multiple positive clones for sequencing to verify the insert...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

...mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional plasmid ...
Tips for CRISPR Gene Editing in Mice

Type

Blog Post

...-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering...
Tips for Writing a Good Cover Letter

Type

Blog Post

... Multiple Authors...
Viral Production at Addgene

Type

Blog Post

...lentiviral vectors. This article was written by multiple Addgenies and most recently updated by Ina Ersing...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

...fluorescent microscope filter sets, users can label multiple proteins of interest with a combination of green...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...bin with a mentor who had attended the meeting multiple times before and would help the Singletons plan...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

...study profiled 38,731 cells from 694 embryos over multiple stages and reconstructed the patterns of gene ...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

... the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced version of PHP.B...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

... so that the marker can be reused to introduce multiple genetic modifications in the same strain. MazF...

Showing: 481 - 500 of 544 results

Results per page:

Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Isolating Genomic Regions of Interest with the CRISPR System

Getting to Know Expansion Microscopy

CRISPR 101: Making a Knock-In Cell Line

Tips for arabidopsis transformation

Great Results Start with Great Standard Curves

Antibodies 101: Producing Recombinant Antibodies

Capturing a Moment with Marc Zimmer’s “The State of Science”

Lentiviral Vector Uses and Overview

Plasmids 101: Colony PCR

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Tips for CRISPR Gene Editing in Mice

Tips for Writing a Good Cover Letter

Viral Production at Addgene

Viral Vectors 101: Preparing Pooled Libraries

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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