Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 863 results for: lic

Showing: 481 - 500 of 863 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...their reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...transmembrane domain receptors, due to the seven alpha helices that transverse the cellular membrane. The receptor...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-... that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also be used...

  7. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...methionine. The labeled proteins can be detected using click-chemistry that covalently links the azido containing... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...

  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement...self-process pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  9. RNA Extraction Without A Kit

    Type
    Blog Post
    ...of DNA that can potentially complicate results of a downstream application such as quantitative PCR to...is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA...

  10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...neuroscience method that lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded... expression in cultured neurons and mouse brain slices collected following intracranial injection of an... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...

  11. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...and in the intersection between science and public policy. Follow David on Twitter @driglar.     References...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    ...the body through the bloodstream, and eventually elicit their actions on other tissues. At the Nuclear ...a broad range of reproductive, developmental, metabolic, and immune response programs. NRs also encompass...gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators Coregulators constitute...

  13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ... when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked by dCas9....for dCas9 to interfere with DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise...doi.org/10.1038/s41467-021-25991-9. Spruijt CG, Gnerlich F, Smits AH et al. (2013) Dynamic readers for ...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

  15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

  16. Tips for Technical Support Calls

    Type
    Blog Post
    ...originally described in a peer-reviewed scientific publication. We love science, but we don’t have the resources...be fairly fast, getting the full answer to a complicated question may take some time. If the customer ...message Answering customer service questions is a delicate balance of knowledge, patience, and diplomacy,...

  17. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...hypomethylation, on the other hand, has also been implicated in the development and progression of cancer ...challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based biosensor...:12404098.  4. Lin, Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically encoded fluorescent reporters...

  18. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ...woodworking tools, and a metalshop. After two summers in Silicon Valley as a student and then instructor at Singularity...

  19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ... and limited the exchange of information to the public and to other academic labs. Being unable to publish... organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry ...something new and beautiful, to engage with the public in a meaningful way about science, and to have ...

  20. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...
Showing: 481 - 500 of 863 results