Addgene

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Showing: 461 - 480 of 509 results

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Corralling and Cornering the Goldilocks Antibody

Type

Blog Post

...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

...from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral Destination vectors. These...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...
Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Type

Blog Post

...Doug Warner on the right) has taught courses in biochemistry, molecular cell biology and genetics at Boston...
Illuminating Epigenetics with A FRET Based Biosensor

Type

Blog Post

...like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...
PhD Applications After COVID

Type

Blog Post

...application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...
Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Type

Blog Post

...., and David R. Liu. "Base editing: precision chemistry on the genome and transcriptome of living cells...
Tips for arabidopsis transformation

Type

Blog Post

...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...

Showing: 461 - 480 of 509 results

Results per page:

Corralling and Cornering the Goldilocks Antibody

Popular Retroviral Vectors and Their Uses in Scientific Research

Adenoviral Vector Production and Troubleshooting

Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Illuminating Epigenetics with A FRET Based Biosensor

Polymerase Chain Reaction Overview and Applications

Hot Plasmids and Viral Preps - May 2021

Cloning Mammalian Cells with the Agarose Method

PhD Applications After COVID

Anatomy of a Plasmid Page at Addgene

Fluorescent Proteins 101: Fluorescent Protein Timers

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Capturing a Moment with Marc Zimmer’s “The State of Science”

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Tips for arabidopsis transformation

Plasmids 101: Secondary Nanobody Toolbox

Tips from the Repository Trenches: Using Barcodes to Track Samples

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