We narrowed to 809 results for: eng
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TypeBlog Post...missing out on an opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an ...
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Your Top Requested Plasmid in 2016!
TypeBlog Post...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...labs around the world. Thank you! It's been a challenging and rewarding journey since starting Addgene.... -
Let There Be LITE Plasmids
TypeBlog Post...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post...available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments. Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...twitter @aigilson. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post... However these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania. References... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by... -
What's New in CRISPR - March 2020
TypeBlog Post.... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...-ME) is a methylation editing tool from Albert Cheng’s lab that enhances RNA-guided methylcytosine editing... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...blog post. References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...trained to be good at learning new things—this is a strength you can draw on and demonstrate in your application...hiring manager. Learn to confidently describe your strengths in your resume and don't be shy about asking for... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post... energy and to choose institutions with allies engaged in training excellence to begin effecting change...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...antibody tentacles may have an impact on this challenge either for magnetic capture or for capture using... a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post... focused on scientific outreach and community engagement. Information about contacting Addgene can be ...office in London, Emma Markham. Emma was born in England but has since studied and worked around the world... -
9 Tips to Achieve Success in Academia
TypeBlog Post... your experiment is definitely not a fluke.” 3. Engage in various projects and help colleagues with their...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...selection screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...cycle proteins, and more. “In the past, genome engineering with CRISPRs was limited to modification of a... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters...genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form ... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...for laboratory waste symposium, sponsored by New England Biolabs and Labconscious. I was eager to learn ...Labconscious. If you’re inspired by this post then I challenge you to take to action! These days, it’s important...