...Jay P. Morgenstern,  Ph.D., Director, Genetic Engineering & Molecular Biology at Warp Drive Bio. The post...Jay P. Morgenstern, Ph.D., Director, Genetic Engineering & Molecular Biology at Warp Drive Bio....

...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...

...details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,...

...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...labs around the world. Thank you! It's been a challenging and rewarding journey since starting Addgene....

...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...

...missing out on an opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an ...

...kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging  to Design: FRET reporter activity depends on...

...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

...available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments.  Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ...

... However these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by...

...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

... energy and to choose institutions with allies engaged in training excellence to begin effecting change...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to...

...antibody tentacles may have an impact on this challenge either for magnetic capture or for capture using... a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl ...

... focused on scientific outreach and community engagement. Information about contacting Addgene can be ...office in London, Emma Markham. Emma was born in England but has since studied and worked around the world...

... your experiment is definitely not a fluke.” 3. Engage in various projects and help colleagues with their...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...

...selection screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...