Addgene

We narrowed to 525 results for: invs

Showing: 441 - 460 of 525 results

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No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

... yeast (109-1010 members). This selection step involves using a commercial kit for in-vitro translation...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

...essentially are messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

... for working with lots of students, managing inventories in the lab, or organizing a research project,...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...
AAVs in Retinal Gene Therapy

Type

Blog Post

...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

... that even though these early steps don’t even involve antibodies, they are still critical to the outcome...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...
A Guide to Starting Your Own Journal Club

Type

Blog Post

... wanted to learn more, even those not directly involved in the project. Once we gauged interest from ...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...interactive. If you can think of a good analogy that involves student participation, make it happen! How should...
Tips for CRISPR Gene Editing in Mice

Type

Blog Post

...supplied by the Zhang lab. This simple website invites you to input the target region (approximately 500bp...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

Showing: 441 - 460 of 525 results

Results per page:

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Plasmids 101: Knockout/Knock-In Plasmids

Plasmids 101: Optimizing Plasmid Yields

Anatomy of a Plasmid Page at Addgene

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Tips for Using BLAST to Verify Plasmids

Transferable Skills Guide: Identifying Your Transferable Skills

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

CRISPR 101: Ribonucleoprotein (RNP) Delivery

AAVs in Retinal Gene Therapy

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

CRISPR 101: RNA Editing with Cas13

A Guide to Starting Your Own Journal Club

SciComm: Taking Science to Elementary and Middle Schools

Tips for CRISPR Gene Editing in Mice

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Tips from the Repository Trenches: Using Barcodes to Track Samples

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