We narrowed to 532 results for: invs

1. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   ...if any secondary antibody remains, whether that involves adding fresh chemiluminescent substrate or imaging...

2. Plasmids 101: Modular Cloning

   Type

   Blog Post

   ...in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule is...

3. Engaging with science and society at pgEd

   Type

   Blog Post

   ...addition to events that we put on, we are often invited to present our materials or speak about our experiences...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   ...produce their shRNA library to support functional investigations of the mammalian genome that can reveal how...

5. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ...complexes, but a serendipitous discovery led them to investigate the use of a common laboratory chemical, guanidine...

6. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   ...is a linear double-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   ...

7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   ...wanted to leave academia, and once she joined an investment club and discovered the business side of science...

8. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ... be a part of it! If you are interested to get involved by submitting data, sending antibodies, and/or...

9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   ...ball-and-chain that they resemble, actually impede your investigation into the regulation of your favorite gene. But...

10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...amount of antibody that binds in the well is an inverse measure of the amount of your protein of interest...

11. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ...policies. Administering Virus Many virus experiments involve needles or scalpels (sharps). Cuts and scrapes ...

12. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ...can be delivered to intact tissues and organs noninvasively and target a defined population of cells (e....

13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    ... for getting a quick summary of papers before investing the time to read them. Organizations: Accounts...

14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ...starting point for SARS-CoV-2 researchers to investigate how the virus interacts with human cells. Addgene...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    ...things at Addgene, each part of this new process involves a significant amount of QC. First, we perform ...

16. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    ...excitation and emission of an excited fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted...

17. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    ... Polymerase II termination is more complex and involves two RNA polymerase-associated proteins, CPSF and...

18. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    ... actually infect cells. These assays typically involve infecting a target cell line with your virus and...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    ...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at...

20. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    ... methods add an additional viral passage. This involves taking your first round of virus, called P1 (or...