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  3. RNA Extraction Without A Kit

    Type
    Blog Post
    ... acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005...Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697...

  5. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...ligated into a vector containing those overhangs. Entry DNA overhangs may be present in the original plasmid... 30-minute reaction. The destination vector and entry vector(s) are placed in a single tube containing...

  7. Hot Plasmids February 2024

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    Blog Post
    ... toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei Shi ...human cell lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely...

  8. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transmits retrogradely to first order neurons, via entry mediated by the G protein/G-protein receptor. Once...powerful tool that can be used to reveal brain circuitry, and continue to contribute answers to the complex...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    ...meetings of experts and leaders from academia, industry, community, education and government; advising...academic venues such as scientific, educational or industry conferences, as well as schools, community events...

  12. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... “cited by” and references sections of a PubMed entry are great places to look for more articles on your...contain your search term. When you view a paper’s entry, the number of citations that paper has is displayed...

  13. PCR Applications for Copy Number Variation Assays

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    Blog Post
    .... 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling in... into thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...

  16. The Challenges of Cell Culture

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    Blog Post
    ...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...

  17. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

  20. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...
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