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  1. Hot Plasmids February 2024

    Type
    Blog Post
    ... toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei Shi ...human cell lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely...

  2. Engaging with science and society at pgEd

    Type
    Blog Post
    ...meetings of experts and leaders from academia, industry, community, education and government; advising...academic venues such as scientific, educational or industry conferences, as well as schools, community events...

  3. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transmits retrogradely to first order neurons, via entry mediated by the G protein/G-protein receptor. Once...powerful tool that can be used to reveal brain circuitry, and continue to contribute answers to the complex...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    .... 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling in... into thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...

  10. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

  12. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...

  13. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...
Showing: 381 - 400 of 509 results