Addgene

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Resume Writing for Non-academic Science Careers

Type

Blog Post

...undergraduate students? On top of that, perhaps you were involved in some extracurricular activities – did you volunteer...
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Type

Blog Post

...methylation-deficient bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...the initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to ...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

...elaborated below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically...
Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

...protomoting or repressing protein expression. Methylases involved with these processes (for example Dam and Dcm ...
15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting...
Early Career Researcher Toolbox: Social Media for Scientists

Type

Blog Post

...open up a new direction for the project. I was invited to participate on a panel about “Social Media for...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

... expression constructs. Sandwiched between two inverted terminal repeats (ITRs) is the promoter and cargo...
Viral Vectors 101: Biosensors

Type

Blog Post

...the system and the receptor, but readouts could involve fluorescence, pH change, heat, etc. The last component...
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Type

Blog Post

...and hypothesis generation, in which data are investigated to look for unexpected trends or differences...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

...collaborative environment for research. We’ve also involved ourselves in extensive outreach programs to encourage...
Behind the scenes: Addgene’s new search engine and more

Type

Blog Post

...cross-team collaboration (thanks to all Addgenies involved). The new homepage and navigational elements help...
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

...which is often over-activated in cancer due to its involved in cell proliferation and differentiation. Since...
AAV Q&A with Tim Miles

Type

Blog Post

...While we have not tried it, it might be worth investigating for both Q6 & Q7.For epithelial cells of the...
Sweating the Small Stuff: Details in the Lab

Type

Blog Post

...rerun an experiment, so consider your prep an investment in accuracy. Bench setup Small adjustments in...
Antibodies 101: Isotypes

Type

Blog Post

...and mast cells, two types of white blood cells involved in allergic reactions. There are no IgE subclasses...
Antibodies 101: Monoclonal Antibodies

Type

Blog Post

...the lab may be their cost. Because of the steps involved in producing and validating monoclonal antibodies...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...components, meaning that the final protocol may involve a sensitive balance of trade-offs. This balance...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

...PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes in mammalian hosts Article...

Showing: 381 - 400 of 525 results

Results per page:

Resume Writing for Non-academic Science Careers

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

CRISPR 101: Non-Homologous End Joining

Screening for Successful Genome Editing with Digital PCR

Plasmids 101: Methylation and Restriction Enzymes

15 Years of Addgene: The Top 15 Plasmids

Early Career Researcher Toolbox: Social Media for Scientists

Viral Vectors 101: Viral Vector Elements

Viral Vectors 101: Biosensors

The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Developing a cold tolerant E. coli using specialized chaperones

Behind the scenes: Addgene’s new search engine and more

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

AAV Q&A with Tim Miles

Sweating the Small Stuff: Details in the Lab

Antibodies 101: Isotypes

Antibodies 101: Monoclonal Antibodies

GCE4All: Making Genetic Code Expansion Accessible

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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