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Showing: 21 - 40 of 54 results
  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... NLS mCherry Connie Cepko 37341 pQC NLS YFP IX Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1...miRFP703 Vladislav Verkhusha 1816 Lamp1-YFP Lysosomes Lamp1 YFP Walther Mothes 1817 Lamp1-RFP Lysosomes...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520... 21741 Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437...
  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...neurons are brightly labeled with GFP (e.g. Thy1-YFP-H mice).  However, we recently developed AAV (adeno-associated...
  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...tdTomato from the CMV promoter pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed...
  4. Bacterial Expression Systems

    Type
    Collection
    ...to the periplasm. Series contains vectors with a YFP variant that functions in the periplasm and can be... FLIParaF.Ec-250n 18855 N/A FRET between CFP and YFP Wolf Frommer Reporter for arabinose levels in bacteria...arabinose enhances the FRET signal from the CFP and YFP fluorophores linked by an arabinose binding protein...
  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Maturation Structure Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz...Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently...moxVenus - Mammalian Expression SYFP2 515 527 69 6 Monomer (A206K) pSYFP2-C1 - Mammalian Expression mGold...Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors...
  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ...templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection markers. Two independent selection...
  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... 2013 Nov 13;7:202. Colin Akerman Chloride (Cl-) YFP-derived sensor for measuring Cl- concentration in...in physiological range A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability...Looger NADH/NAD+ Fluorescent biosensor of NAD/NADH (RexYFP) Genetically encoded fluorescent indicator for ...
  8. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... serotype AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) AAV PHP.eB Controls ...viral prep AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) AAV PHP.eB Controls ...New viral prep AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) AAV PHP.eB Controls ... viral prep AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) AAV PHP.eB Controls ...viral prep AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905) AAV PHP.eB Controls ...Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New serotype ...
  9. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype...mScarlet-KV2.1 55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R)-EYFP 26975 AAVrg pAAV-CaMKIIa-hChR2(H134R)-mCherry ...H134R)-mCherry 26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP   Control AAV Control AAV allow researchers to...Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP   Biosensor AAV...pAAV-hSyn-DIO-mCherry 27056 AAVrg pAAV-Ef1a-DIO EYFP 114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2...
  10. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-mcherry) ...expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-... Figure 2: Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry), under the ...
  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...to access them! Controls pAAV-hSyn-hChR2(H134R)-EYFP (26973-AAVrg) pAAV-hSyn-hChR2(H134R)-mCherry (26976...AAVrg) Optogenetics pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (55645-AAV5)  Additional resources on the Addgene...
  12. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... demonstrate the power of mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in...
  13. Hot Plasmids - October 2022

    Type
    Blog Post
    ...+ and other cations. B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer 2/3 neurons in mouse...
Showing: 21 - 40 of 54 results