New Tools Enable CRISPRa for Neuroscience Applications
Type
Blog Post
...genetic underpinnings of these processes has been complicated by the lack of precise genetic tools for modulating...recent advancements and offer commentary on their application to neuroscience research.
What is CRISPR-based...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly...necessitates such molecular gymnastics for sensitive applications such as CRISPR screening.
The plasmids used ...used by the Qi Lab for this particular application can be found here.
Why CRISPRa?
Although cDNA overexpression...gene activation in select in vitro and in vivo applications, the tools described above make CRISPRa in the...relatively easy and accessible. There are also other applications for which CRISPRa is well-suited, including ...