...Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening...

...Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila Plants...

...subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first...

...looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus pyogenes.   Figure 2:  Crystal structure...

...can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease...Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola...

...monocytogenes  pCSW21  Yes  pJH376 Yes   acrIIA3 Streptococcus pyogenes  pCSW24  N/A (Toxic) pJH375  No  ...

...can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease...Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola...