...transgenic mice. ID Plasmid/Article Description Expression System PI See article Brainbow constructs Cre-...UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian van Rheenen...activates your gene fused to eGFP, removes dsRed. See article for other similar plasmids. Retroviral Clevers ...with multiple distinct colors Mammalian Sanes See article Multibow constructs Multibow uses a binary code...vector, Cre dependent tdtomato expression. See article for more colors. Mammalian Zeng 24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean...

...which enable the production of infectious AAV particles without the use of a helper virus. Instead, specific...produce AAV. In order to generate infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A...and/or can be used to generate infectious AAV particles. Additional helper plasmids (which may not be ...Addgene) may be required to produce infectious AAV particles. You can search or sort this table based on plasmid...

...select the article link to find more information. Delivery Method Species Description Article PI Lentivirus...the article link to find more information. Convert From Convert To Delivery Method Species Article PI ...

... Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list...Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , February...the Zhang lab has published this Nature Protocol article . Should I add the PAM sequence to the oligo when...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...publication link to view all other plasmids from that article. Click on the PI link to view all available plasmids...

...about the repression system can be found in this article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard...chromosomal integration of pLC97 can be found in this article: St-Pierre, F., Cui, L., Priest, D. G., Endy, D...

...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Order a high-quality... examples of quality control data. Help Center Articles Additional details about viral vector production...

...mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide for more information...pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV...

...control). The end result of the meeting will be a publicly shared practical guide for all scientists in the...have final approval before anything is released publicly, but please only share materials that you are ...

...generally represents the physical number of viral particles. Users may need to run lower or higher dilutions...

...filters made of materials other than PES. AAV particles stick to many other surfaces, but do not stick...

... are not efficiently packaged into lentiviral particles. See below for options if the recombinant band...

...provide the necessary components to make lentiviral particles (for details about lentivirus, see our Lentivirus... AAV transfer vector and used to generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging...

...Featured article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell...download the full-text of the publication here . Article Citation Generation of Genomic Deletions in Mammalian...

...saw that these AAV particles come in a slightly different shape. These AAV particles are often found with...with cellular exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are... found that the association of AAV particles with membrane vesicles protects them from antibody recognition...exosome AAVs is related to the use of microvesicles as a vehicle for drug delivery that has recently gained...During vector production, assembled AAV vector particles accumulate inside the producer cells and purification...process that begins with cell lysis to release AAV particles with subsequent purification steps. It’s also ...cellular membrane into the culture medium. AAV particles are either associated with the outside surface...

...getting these particles to infect cells is also very titer-dependent. In order for an AAV particle to infect...causing less particles to be released into suspension and lowering the number of viral particles that can ...generating viral particles. Limit tube transfer - the fewer opportunities your viral particles have to stick...contact with a cell Although an individual AAV particle’s ability to infect a cell is dependent on its ..., you can see the dwindling proportion of AAV particles that make it through each part of the cell on ...expressed. The bottom line is that a lot of AAV particles are needed to get a few good ones into a cell ...contact between the cell and any individual AAV particle is rare. But simply getting into the cell may ...

... gas vesicles gene clusters from a diverse set of gas vesicle-expressing species. The gas vesicle genes...flos-aquae gas vesicle gene cluster because of its small size and its ability to produce gas vesicles with nonlinear...of air-filled protein nanostructures called gas vesicles, which scatter sound waves and thereby produce...pBAD-bARGSer-AxeTxe (Fig. 1a).  With pBAD-bARGSer-AxeTxe, gas vesicle expression in E. coli is easy: simply grow cells...arabinose-containing plates will be visibly white, and gas vesicles in cells are visible under both phase contrast...microscopy and electron microscopy (Fig. 1b). Gas vesicle expression in vivo is also straightforward: after... before imaging with ultrasound to observe gas vesicle-specific ultrasound contrast where the bacteria...

...by the ratio of physical viral particles to infectious viral particles. Ratio of full to empty viral capsids...vector particles. This image shows that the vast majority of the vectors consist of full particles (white...? Physical titer: the concentration of viral particles containing viral genomes. Physical titers are ...quantification methods - see below), since each viral particle typically contains one viral genome. Other than... Infectious titer: the concentration of viral particles that can transduce cells. Infectious titers are... to have a near-perfect physical-to-infectious particle ratio of 1:1 (Zeltner et al., 2010). However, ...the same study reported a physical-to-infectious particle ratio of 50:1 (Zeltner et al., 2010). The specific...

... be integrated into the new viral particles and the virus particles produced could not replicate. However...favourite cell line or into primary cells, this blog article will give you some tips to plan your experiment... genes necessary for the production of a viral particle (Gag, Pol, Tat, Rev and Env) and the transfer ...necessary to encapsulate that insert into a viral particle (the packaging signal Psi). Because the packaging...and broadens the cellular tropism of the viral particles produced. However, in this vector production system...essential for the production of the lentiviral particle have been removed. This generation is safer than...set-up and purify and concentrate your lentiviral particles to get them to the optimum concentration to infect...

...proteins that form bacterial gas vesicles. These ~200nm long vesicles have hollow interiors and a protein...and arg2 vesicles is the amount of soundwave pressure required to collapse them: arg2 vesicles collapse...collapse at a lower pressure than arg1 vesicles. Following collapse, vesicles are invisible to ultrasound, which...(ARGs) overcome these limitations by using gas vesicle reporters that are detectable by an inexpensive...that excludes water but is gas permeable. These vesicles are primarily used by water-dwelling photosynthetic.... The Shapiro lab recently discovered that gas vesicles also scatter sound waves and therefore produce...resolution. By piecing together genes from gas vesicle operons from two different bacteria, Bourdeau et...