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Showing: 21 - 40 of 204 results
  1. New Coomassie Protocol Video

    Type
    Blog Post
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...
  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Verkhusha Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow...superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein...mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins...requires some time for chromophore maturation, splitFAST displays rapid and reversible complementation....
  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to...some of the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...case, consulting can sometimes be challenging. The fast paced nature of projects often means working long...
  4. Hot Plasmids - October 2022

    Type
    Blog Post
    ...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...
  5. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...characteristic to capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...sample is then collected and projected back to a fast, sensitive detector such as a scientific CMOS camera...planes of a sample may also be useful for providing fast imaging along with optical sectioning (5). One such...preferred technique for many of these samples due to its fast temporal resolution and decreased phototoxicity....captured in a single camera exposure. This is much faster than relying on raster scanning as is done in confocal...
  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...transfer at the subcellular level requires a probe with fast kinetics and high signal/noise ratio. Here, Takeharu...Figure 7: B-gTEMP temperature-sensitive probe enables fast measurement of temperature and local heat transfer...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity...
  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. ...to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic...
  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...Created with BioRender.com. Introducing ABE8e - a fast and flexible adenine base editor Lower-stringency...resulting base editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct...The idea was to evolve a TadA-7.10 version with a faster deamination rate so that deamination can happen...PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems allow for rapid continuous...speed dictates which phage variants can propagate faster than the rate of dilution (passage from the infected..., they identified TadA-8e, which edits 590-fold faster than TadA-7.10. It is compatible with all Cas9 ...functional domains speed up the development towards faster and more precise base editors capable of editing...
  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...example, the far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal ...surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a tdTomato fluorescent...in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... Alter chromatin architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing...
  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...
  11. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...CRISPR plasmids New items from the viral service A fast blue-light inducible Cre for precise control of ... can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours.  Sheets, MB...
  12. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Highly similar sequences (megablast)”, which is very fast and works best when the identity between your sequence...base pair from your results. This option is not as fast as megablast, but can return longer alignments to...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ... [Our QC process would be trouble-free and much faster if 95% of our results were always correct!]  Since...
  13. Optogenetics Guide

    Type
    Guide
    ...foundational optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light.... Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Nat ...compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude 490...
  14. CRISPR Cheat Sheet

    Type
    Blog Post
    ...CRISPR material for you!  The CRISPR field moves fast. That’s why we’re bringing you a brand new third...
  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...these viruses. The cells are also easy to cultivate, fast-growing, and widely available. The viruses Lentiviruses...like HEK-293T cells, they are easy to cultivate, fast-growing, and widely available. Unlike mammalian ...
Showing: 21 - 40 of 204 results