.... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...

...the Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells...Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege ...expression backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter...rather than a mixed sample. TRIBE was developed in Drosophila, and further work is needed to assess its function...plasmids optimized for dCas9-VPR expression in drosophila and yeast. Chavez A, et al. Nat. Methods 2015...

...number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria...

...increases knock-in efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation...PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https...

...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

...Fragmid was developed for mammalian systems and Drosophila (McGee et al., 2023). Fragmid developers noticed...described above works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate...

...genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light is another way...

...Is there any AAV serotype that can be used in Drosophila? A: Not to our knowledge. Disease Applications...

...includes systems for tagging mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating...

...which it was previously not an option, including Drosophila and C. elegans.   Find the TurboID plasmids here...

...GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and jGCaMP8...

...an open science genome engineering project in Drosophila. ...fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and ...1gRNA expression of gRNA under control of the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression... expression of gRNA under control of the Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression...expression of gRNA under control of the Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs ...3tandemgRNAs expression of two gRNAs from Drosophila U6:1 and U6:3 Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock...SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. Bullock 62208 pnos-Cas9-nos Expresses...

...can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept...

...by members of the worm community but there are Drosophila and zebrafish articles published there as well...

...your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon,...

... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

...sources – voltage-gated potassium channels found in Drosophila to phosphatases from sea squirts. This design...

...genes: deletion mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002...

...Washington University whose research focuses on Drosophila-parasite pathogen interactions.  ...