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  3. Plan Your Experiment

    Type
    Collection
    ...dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression of an endogenous gene(s) without...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming...

  5. Plan Your Experiment

    Type
    Guide
    ...dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression of an endogenous gene(s) without...

  6. CRISPR Guide

    Type
    Collection
    ...CRISPR interference, or CRISPRi) or activation (CRISPRa) of downstream target genes can be achieved by ...bacterial genomic region used in pathogen defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator...protein-interacting RNA aptamers, such as in the CRISPRainbow kit . Using multiple copies of the aptamer improves... loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (5), 528–530. PMID...

  7. CRISPR Guide

    Type
    Guide
    ...CRISPR interference, or CRISPRi) or activation (CRISPRa) of downstream target genes can be achieved by ...bacterial genomic region used in pathogen defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator...protein-interacting RNA aptamers, such as in the CRISPRainbow kit . Using multiple copies of the aptamer improves... loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology . 34 (5), 528–530. PMID...

  8. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...guide RNA sequence. A year later, they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that...three hairpins produces a white color. While CRISPRainbow allows for simultaneous visualization of sites... are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted... of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they knew they could be good candidates for...performed, they compared them head-to-head against CRISPRainbow. Both tools were targeted to the FBN3 intronic...noise ratio can be increased from 2-fold (by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color ...used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable...
Showing: 21 - 32 of 32 results