...multimerization of the PCR primers can cause duplication of the primer sequence in the resulting plasmid...proximal to the target site, can identify these duplications (slightly larger bands sizes relative to the...percentage agarose gel (~3%). Note that primer duplication will evade detection in the initial RFLP analysis...because the introduced restriction site is also duplicated. Validating your mutagenized plasmids Once constructs...identified (and confirmed to be free of primer duplications), final validation is performed by sequencing...

... for PhDs or postdocs. Having this information publicly available may realign trainee expectations with...Publishing Panel brought in editors from a variety of publications as well as young PIs and all gave advice on ...blogging, write reviews, or write for a university publication, improving your general writing skills will help...cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing it into a black...Science The Future of Research Website Additional Publications of Interest Can Be Found Here Resources at ...

...adapted from Andrews et al., 2019 under CC-BY 4.0 license.   Selecting your antibodies When selecting...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...978-1-4939-8935-5_26 Jambor, H., Antonietti, A., Alicea, B., Audisio, T. L., Auer, S., Bhardwaj, V., Burgess...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi....Immunocytochemistry Protocols for other antibody applications...

...within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the plasmids...account on PubMed. Once you have an account, simply click on your account name in the top right corner of ...PubMed to navigate to your account settings. Next, click on the link in the “NCBI Site Preferences” box. ...Preferences page, navigate to “PubMed Preferences” and click on the “Filters & Icons” link (see image to the ...

...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein...

... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...

...funding and volunteer opportunities, protocols, publications, and more to accelerate the pace of research...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely fashion.... a significant impact on both clinical care and public health for this current COVID-19 pandemic, and ...

...modified the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...

...under harsh conditions. One example application from the publication is the removal of unwanted cDNA derived...arrange permits to visit places not accessible to the public and retrofitting our car with a freezer wired to...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...

...experiments The Tian lab used dLight1 both in brain slices and freely behaving mice to gather the following...and concentration of released DA in mouse brain slices Visualization of DA release dynamics during locomotion...GRABDA in drosophila, zebrafish, and mouse brain slices and freely moving mice to gather the following ...from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection in a single neuron in ...

...the gRNA design process and rules for various applications is necessary going forward. The standardization... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2...for CRISPR Genome Editing Other resources and publications CRISPOR gRNA design tool Researchers at the ...

...developmentally programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis...be other scientists, or science journalists, or policy makers. The term editor covers a wide variety of...access affects financial models in information publication. Take a look at the blog ‘Scholarly Kitchen’ ...International Congress on Peer Review and Scientific Publication, where diverse approaches to peer review were...

...containing only the sequences required for viral replication and transcription as well as reporter genes. ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action...these libraries from the post and the associated publication. Optimizing Donor DNA for Enhanced CRISPR Genome... to your DNA sequence of interest. Potential applications of the system include activation and repression...

... to help decide whether work is suitable for publication in their journal. The early peer-review attempts...information that would prevent the study to be replicated or omission from the literature. I think that.... The code and the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare...Addgene.org Learn about depositing plasmids pre-publication Find molecular biology protocols Find eBooks ...

... (AmpR) and origins of replication (ori and f1 ori) which allow it to replicate.   How can I prevent...the bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...

... and written communication skills are key to publication and acquisition of funding. If a grant committee...editing manuscripts? Are you interested in scientific policy? Next, use the resources below to identify potential...Understanding)   Writing Communication Publicity Consulting internships Communication...Blogging Writing Communication Networking Publicity Business competitions   Leadership ...

...protein-protein interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...utility of these FLiPs in a variety of research applications and particularly notes their ability to be precisely...Update (7/17/2018): Check out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional...

...databases available, Web of Science. For each publication, Web of Science provides basic bibliographic ...Science, PLOS One, and PNAS. We then broke the publications from these journals into two groups: those containing...differences in citations due to the journal of publication. Table 1 provides an example calculation of the...Addgene in them. The adjustment is done within publication years. What do we find once we do this adjustment...