...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...

...these vectors for gene therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector... vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr...

...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...

...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...hottest antibodies in Addgene’s collection since we began distribution and has been verified to perform well...

...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...

...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...

...techniques are also being applied to study model organisms such as Drosophila and zebrafish. Brainbow in ...important insights into the complicated physical organization of the brain and other biological systems. Interested...

...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...

...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...

...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...

...post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira... Students also need more access to reputable, organized networks connecting international experts and ...

...-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT ...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA...

...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...

...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn...

...expensive, and not to mention frustrating. One elegant solution to sidestep the antibody problem is to...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant ...

...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...

...transdifferentiation factors are directly injected into the organ of interest for in vivo transdifferentiation. Multiple... Williams, L.A., B.N. Davis-Dusenbery, and K.C. Eggan, SnapShot: directed differentiation of pluripotent...

...acquired from the the fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent...