...synthetic biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's...projects in Ghana.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

... the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria) for...grow with his help! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

....     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called...efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases. In doing so, they ...created a modular toolkit for Cas-mediated genome engineering in plants. Read the paper in bioRxiv Find...

... up with it. The peer review process has been challenged with many closely spaced CRISPR software submissions...facilitate most experimental applications in CRISPR engineering. When compared to that collective utility, it...This is only one of many decisions faced by those engaging in CRISPR design, but it is a significant hurdle...limitation, but the tool is fast enough that, if you’re engaged in heavy CRISPR design (I’m talking screens and...

...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed...PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries...

...My son is an engineer and my daughter has a business degree. From the very start of their undergraduate...talent in science related careers. Failure to strengthen the talent pipeline sets the world up for reduced...Image from pd|hub.   To begin to tackle these challenges, a group of stakeholders came together with the...

...concatemers, are spliced together, and the full-length transgene can then be expressed. This method allows...homologous recombination and expression of the full-length transgene at very low efficiency (<1% of wild type...therapy: Designer gene delivery vectors: molecular engineering and evolution of adeno-associated viral vectors...vectors: a quantitative comparison. Duan D, Yue Y, Engelhardt JF. Mol Ther. 2001 Oct;4(4):383-91. PubMed . ...

...genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...activation-induced cytidine deaminase (AID) and an engineered tRNA adenosine deaminase (TadA) to a catalytically...packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable ...

... some challenges during my internship, but nothing could have prepared me that those challenges would ...exploring my different interests but also a way of challenging what I am used to. Brandon Solis, marketing...

...be removed by re-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic ...the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s replication...clones tested had plasmid integration. Another challenge is that multiple transfection makes it difficult...induced pluripotency: Recent progress and future challenges." Journal of Cellular Physiology 228, no. 2 (...

...co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently working...interview. I anxiously prepared for the call. The length of this interview was intimidating to me, as I ...comfortable.     Lucie Wilson is a rising junior and bioengineering student at Northwestern University. She is ...

...researchers involved. Communication is a major challenge when researchers are communicating across disciplines...disciplines or doing different tasks. But these challenges can be exaggerated when communicating across ... help other teams successfully overcome these challenges, too. Schedule regular meetings Scheduling a...

...them attractive tools for the life scientist or bioengineer.   Figure 1: Map of a plasmid with its...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

...fluorescent protein-based sensor. This sensor was engineered from a GFP variant and a DA receptor such that...PMCID: PMC3083031. Kebabian, John W., and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible ... Kebabian, John W., Gary L. Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate...

...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

...Kurt Swanson a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made... Swanson is a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in...

...the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering and experimental ...the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin...

.... The resultant JEDI-2P sensors overcome many challenges in 2-photon in-vivo imaging of voltage fluctuations...neurons, far out-performing previous attempts to engineer potassium-selective channelrhodopsins. HcKCR1 ...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...

...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-...

... fiber (Park et al., 2019). This provided an engineering road map of sorts, giving an idea of the stiffness...whenever necessary. This is all an impressive engineering feat, but you’re probably wondering “did it actually... Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), 4322–4325...