Skip to main content
Addgene
Showing: 341 - 360 of 509 results
  1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’... (i.e. RNA replication with no proteins). The chemistry is complex and this work requires an interdisciplinary...forms as we shared millions of years of common ancestry with worms before evolutionary divergence. Nikolaus...
  2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...
  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...
  4. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...
  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...
  6. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...
  7. Hot Plasmids - November 2023

    Type
    Blog Post
    ...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...
  8. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...
  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...
  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...
  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...
  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...the top right of each data entry page. Once you have completed the data entry process for all of the plasmids...Request Deposit Kit.” button to complete the data entry. Clicking this button will allow you to choose which...
  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...
  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ... if an export license is not required for your country after checkout, and the processing fee will be ...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...
  15. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...
  16. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition, and...snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided...
  17. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...date, material, requesting PI & organization, and country. This information can be of personal interest to...horizontal bar chart) Number of requests per country (as dot plot) Number of requests per year (as...
  18. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ... data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of the Supreme...factor, study topic, and the corresponding author's country of origin, were taken into account. 7)     Science...
Showing: 341 - 360 of 509 results