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TypeBlog Post...cells. The cells then get to work fixing up the “damage” and producing a complete plasmid. Besides being...
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A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...data sets include videos of behavior, many many images of brain regions, or sequences of microbial DNA... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...can produce some very beautiful immunofluorescent images like in this paper (2). Metabolic assays such as... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...interactions (Ref 3). You can find useful tips on in vivo imaging in our Fluorescent Protein Guide. Throughout the... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...also have the ability to to download the static image file (PNG, see above) for your own reference or ... -
Viral Vectors 101: Viral Applications
TypeBlog Post...single dose. Vaccine development strategies. Image courtesy of Gorry et. al, 2007. Clinical... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post.... 1: Viral particle infection into a host cell. Image courtesy of National Human Genome Research Institute... -
AAV Q&A with Tim Miles
TypeBlog Post...individual is reported in Figure 4B (see left for image and link for paper.) Q12: Is there any AAV serotype... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...grind of science. References and Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post... Figure 1: Result of a blue-white screen. Image from Stefan Walkowski. Restriction digest Another... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...don’t respond as expected, use of representative images, measuring the main outcomes in several different... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...recombineering and Multiplex Automated Genome Engineering (MAGE) will always be limited by the size of the library... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...synbodies--or synthetic antibodies--attached to magnetic beads, allowing the virus to be easily concentrated... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...his-tagged OptoNBs. OptoNB coated beads were then imaged while cycling between dark and blue light which... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post... place, Konermann immediately saw an order of magnitude improvement in activation and knew she was on ... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...synonymous with GM to the point that people cannot imagine any use for GM technology other than to sustain... -
Antibodies 101: Fab Fragments
TypeBlog Post...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot... -
Antibodies 101: Conjugation
TypeBlog Post...chemical reaction, can be detected using specialized imaging instruments or X-ray film and lends itself well... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...experiments, Clift et al. observed no stress or damage upon TRIM-Away, and the electroporated cells re-adhered...