Tips for a 1st time AAV user (by a Rookie AAV user)
Type
Blog Post
...which provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase...adenovirus genes which help mediate AAV viral replication; and 3) the transfer plasmid which encodes a ... that aids in the homologous recombination of duplicated regions like the ITRs. Restriction digest screening... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...