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Showing: 341 - 360 of 590 results

  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

  3. Lab Automation at Addgene

    Type
    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    ...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which will...target protein and an E3 ligase through interacting ligands. After an E3 ligase facilitates ubiquitination ...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...

  18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which result in the internalization...allowing for ligation to pyridyl disulfide-activated ligands. Due to the concerns of off-target mutations and...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein...soon as its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis...

  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...
Showing: 341 - 360 of 590 results