...while flow cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry...signaling molecule has a weak signal. For flow cytometry, secondary antibodies may be indicated on occasion...assumption is incorrect. With the exception of flow cytometry, most immunoimaging assays do not allow for absolute...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

...However, internationalization, the ability to cross country boundaries for both professional and personal development...boundaries and should not be confined by birth country or financial means. Instead, talent should be realized...talented professionals (who migrated from their country some years ago looking for their career improvement...bright students that will be the future of the country. Brain drain is a serious problem in several countries...immediate frontiers. Moreover, sharing a culture/country of origin with an international mentor creates ...University of Navarra, and the University of the Basque Country. Mentees represented a diverse group of individuals...

...and even some you’ve likely never heard of. Each entry displays the following information for a Cas enzyme...display both the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently... 2 – List of resources and properties for a Cas entry (SpyCas9a) from CasPEDIA.   How can I use it...RefSeq ID, etc.) and you will see a table of protein entry results. For the advanced search options, this is...System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology. https:/...

...an important role in viral entry, which are inhibitors of SARS-CoV-2 entry and become upregulated during...plasmids, with many available to scientists in industry. Since March 2020, we have received over 13,000... which human genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al....glad to join these efforts and help academic and industry scientists through plasmid sharing and more. As...

...the most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send us your...information in a deposit spreadsheet. The data entry process On the Addgene home page, if you scroll ...top of the page, you’ll see two options for data entry: “Submit Online Deposit” and “Download Deposit Spreadsheet...blog post for a thorough guide to our online data entry process. Figure 1: Begin the deposit process by...deposit spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries...

...create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used... various constructs with applications in flow cytometry approaches in mammalian cell culture systems (...deoxyribonucleic acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780...Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015...deoxyribonucleic acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810...

... contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do ...guest blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing...progress and prospects." Journal of Biological Chemistry 284.26 (2009): 17365-17369. PubMed PMID: 19329793...ligase reaction and bead enrichment." Analytical biochemistry 434.1 (2013): 34-38. PubMed PMID: 23142428. ...of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed PMID: 24328157. 21...

...application — flow cytometry, western blot, immunohistochemistry, immunocytochemistry, ELISA — but in all...antibody data report, showing the results of an immunocytochemistry experiment performed with the anti-Lhx6.1...

... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple... seminars, such as those in bioinformatics or chemistry, even if your primary focus lies within a biological...successful research career, whether within academia or industry. Albert Einstein once said: “There are only two...

...Fighting with antibodies to produce immunohistochemistry images that are crisp, bright, and lacking in...mouse-on mouse” (or any “species on species”) immunochemistry (IHC) applications an issue? The culprits are...avoiding background secondary binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your...impact other techniques like Western blots or immunocytochemistry (ICC). Not all of the solutions described...

...am heading into my third year of college as a biochemistry major and a Spanish minor, it’s time for me ...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...the Addgene blog. A few of my friends in the Biochemistry major seem to have the idea that if you don’...of Business Development, got her PhD in Plant Biochemistry in France. When she moved to the U.S., she discovered...

...validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important to note that...compatible with your conjugate and conjugation chemistry.  Quantification Once you’ve collected your antibody...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ...

...interest. In brief, the insert is first cloned into an entry vector at a region flanked by sequences (called ...

...used rAAV FLEx-based vectors to study the neural circuitry behind the mechanisms of sleep and anesthesia....Cre recombinase protein." Journal of Biological Chemistry 259.3 (1984): 1509-1514. PubMed PMID: 6319400....behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157. PubMed PMID... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

...immunoprecipitation, immunohistochemistry, immunocytochemistry, and flow cytometry. It's a great introduction...

...a desired component are cloned into Golden Gate entry vectors via traditional restriction enzyme cloning...cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden Gate cloning step to...to generate the final construct. Up to eight entry vectors can be used for each Multisite Gateway compatible... Production group at Max-Planck Institute of Biochemistry has developed a number of expression vectors..., and plant resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination...

...genetic circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic...shuttle, our driver regaled us with tales of back country skiing, fly fishing, and local grizzly bear attacks...

... science began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with...do,” Stegmeier says He began this journey in biochemistry made the foray into genetics during graduate... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...

... like serotype, promoter, or tool type and each entry contains an image along with key experimental details...to input your data for sharing on the site. The entry form has 5 general question types and it takes approximately...use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment...

...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...uses it to match qualified scientists to jobs in industry, academia, and government. This great resource... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...