We narrowed to 390 results for: promoter
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TypeBlog Post...from a replicating adenovirus using native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016...
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Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...binding and contributing to DNA unwinding. This promotes initiation at the plasmid ori, after which replication... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...localization sequence) and expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...interest. A low primer-to-template ratio is used to promote annealing of one primer to each template. The PCR... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
10 Steps to a Perfect Science Talk
TypeBlog Post...non-interview setting. There are many resources on self-promotion (how hard it is for some people, especially women... -
Site Directed Mutagenesis by PCR
TypeBlog Post... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...reinforces behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation... -
Synthetic Biology - Assembly Standards Guide
TypeCollection.... Therefore, the same reaction which ligates a promoter into an empty backbone can be repeated to ligate...ligate your gene of interest after the promoter, and so on. The following table provides information for... -
Control AAV Preps
TypeCollection...that express fluorescent proteins under general promoters. These AAV can be used to compare the activity...PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants...Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...When you go on interviews or are looking for a promotion, have some solid examples of times when you have... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but... -
Lentiviral Prep Service
TypeCollection...Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories...3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service Suggestions Don’t... -
Synthetic Biology - Sensing and Signaling
TypeCollection...depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for... -
Zhang Lab CRISPR Page
TypeCollection...Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; for sgRNA...tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a U6-driven single guide RNA. The vector ...codon-optimized SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in... -
Synthetic Biology - Metabolism
TypeCollection...depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back to Top Do you have suggestions... -
Zinc Finger Consortium Reagents
TypeCollection... The Zinc Finger Consortium was established to promote continued research and development of engineered...