We narrowed to 396 results for: promoter
-
TypeBlog Post... difference between them lies in the choice of promoter for ωRNA (reRNA) expression (see Figure 1). Both...
-
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
10 Steps to a Perfect Science Talk
TypeBlog Post...non-interview setting. There are many resources on self-promotion (how hard it is for some people, especially women... -
Site Directed Mutagenesis by PCR
TypeBlog Post... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...reinforces behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation... -
CRISPR Plasmids - Xenopus
TypeCollection...specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed... -
CRISPR Plasmids - Zebrafish
TypeCollection... than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed... -
Brain Armamentarium
TypeCollection...Viviana Gradinaru 163489-AAV1 AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA Direct-expressing oNigri AAV...pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-B22 ...pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS1...pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS2...pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru... -
Optogenetics Plasmid Collection
TypeCollection...ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Return to top ...ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to top Content last reviewed: 25 September... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm ...of two sgRNAs from independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for...along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate vectors and then... assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one...pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs...expression of two gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous ... -
Serotype Testing AAV
TypeCollection...Boyden ) and direct GFP expression from the CAG promoter. For information about each catalog item, including...direct EGFP expression from the human synpasin promoter. For information about each catalog item, including... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...When you go on interviews or are looking for a promotion, have some solid examples of times when you have... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use a single polycistronic... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but... -
Plan Your Experiment
TypeCollection...template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency...prime editing. CRISPR Intereference : Target the promoter region of the gene of interest, where the Cas9...approaches, the species and expression pattern of the promoter for both your Cas enzyme and gRNA and the presence...the wide range of options for the Cas enzyme, promoters, and selection markers. There are also no packaging...to specific cell types by using cell-specific promoters, which is useful for in vivo studies. While we...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned... -
Synthetic Biology - Assembly Standards Guide
TypeCollection.... Therefore, the same reaction which ligates a promoter into an empty backbone can be repeated to ligate...ligate your gene of interest after the promoter, and so on. The following table provides information for... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene. Here, the target window is not... -
Hot Plasmids: Summer 2024
TypeBlog Post...sites on the target DNA, and thus inactivating the promoter. Image reused with permission from Neumann et ...