We narrowed to 567 results for: multi
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TypeBlog Post...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...
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CRISPR 101: Validating Your Genome Edit
TypeBlog Post... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for... Multiple Authors... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...suboptimal folding or stability. Thus they conducted multiple rounds of directed evolution until they identified...detect RNA-protein interactions that can regulate multiple biological functions. RapID (RNA-protein interaction... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different... AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross... -
CRISPR 101: Off-Target Effects
TypeBlog Post...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...and reprobed with different antibodies allowing multiple targets to be assessed from a single western. ...used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the primary ... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post... genome amplification (by a mechanism known as multiple displacement amplification) even in the presence... of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order to reduce... -
Plasmids 101: Restriction Cloning
TypeBlog Post...of the promoter as part of a multiple cloning site (MCS). Having multiple sites allows you to easily orient... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...techniques. These include reusing pipettes for multiple cell lines rather than using disposable ones or...mycoplasma infection from one cell line to another. Multiple cell lines should not be stored in a single incubator... -
Important Considerations When Using AAVs
TypeBlog Post...you can see, AAVs of each serotype can transduce multiple tissues. So how do you choose the right serotype...thawing. Although more stable than other viruses, multiple freeze thaw cycles can still reduce AAV titer.... -
Antibodies 101: Validation
TypeBlog Post...antibody works! Multiple (Independent) Antibodies Strategies These strategies use multiple, unique antibodies... -
9 tips for a successful postdoctoral experience
TypeBlog Post...a Group Leader position, what will you need? Multiple first or corresponding author publications (published...interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad... -
Antibodies 101: Flow Cytometry
TypeBlog Post...be beneficial when expression levels are low as multiple secondary antibodies can bind to a primary antibody...causing dead cells to fluoresce. When running a multicolor flow cytometry experiment, users must also prepare... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...reprogramming factors can be delivered and expressed in multiple somatic cells via various methods. Some of the... of interest for in vivo transdifferentiation. Multiple cell types such as fibroblasts, hepatocytes, and... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...conversion in principle, but some changes require multiple steps and enzymes. Prime editing can encode any... Multiple Authors... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...modifications. CRISPR base editing persists through multiple cell divisions, indicating that this method produces... Multiple Authors... -
Protocol - How to Perform Sequence Analysis
TypeProtocol... you can see that just after base 70 there are multiple peaks in the same location. Looking at the trace... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...J., Deverman, B. E., & Gradinaru, V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs ... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...