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Showing: 321 - 340 of 567 results
  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...
  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...
  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different...  AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross...
  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ...
  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...and reprobed with different antibodies allowing multiple targets to be assessed from a single western.  ...used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the primary ...
  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... genome amplification (by a mechanism known as multiple displacement amplification) even in the presence... of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order to reduce...
  7. Important Considerations When Using AAVs

    Type
    Blog Post
    ...you can see, AAVs of each serotype can transduce multiple tissues. So how do you choose the right serotype...thawing. Although more stable than other viruses, multiple freeze thaw cycles can still reduce AAV titer....
  8. Antibodies 101: Validation

    Type
    Blog Post
    ...antibody works! Multiple (Independent) Antibodies Strategies These strategies use multiple, unique antibodies...
  9. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...a Group Leader position, what will you need? Multiple first or corresponding author publications (published...interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad...
  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...be beneficial when expression levels are low as multiple secondary antibodies can bind to a primary antibody...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...
  11. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...
Showing: 321 - 340 of 567 results