RNA Interference in Plant Biology: New Tools for an Old Favorite
Type
Blog Post
... naked and unmodified dsRNA elicits immunogenic reactions that complicate the effectiveness and interpretation...silencing can be easily induced by exogenous application of long or short dsRNA. However, it is important...have benefited from the ease of use in dsRNA applications.
Figure 1: Schematic of an RNAi-inducing...Historically, gene silencing methods were thought to be afflicted by pervasive off-target effects. This conclusion...LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning.
Artificial ...