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  1. March for Science

    Type
    Blog Post
    ...should not effected by claims that ‘facts’ are subjective or alternative. Inclusion of data informing...informing policy specifically related to the following subjects: Climate change Vaccines GMOs Transgender issues...

  2. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic archea: these organisms lacked ...phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express the completely...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...resulted in appropriately localized dystrophin. In a subsequent study, they used CRISPR with two gRNAs to delete... mRNA is high, likely because this mRNA is not subject to nonsense-mediated decay. Although Nelson et ...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...only includes general lab strains designed for subcloning or protein expression. If you were to include...exogenous proline source for growth rpsL Mutation in subunit S12 of 30S ribosome Confers resistance to streptomycin...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Datatable. A more extended discussion of these subjects can be found in two recent review articles (Doench...involves the need for single cell cloning and subsequent screening for successful edits. This is a very...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...setup. A light source delivers light pulses to the subject/cells expressing the optogenetic tool causing a...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... don’t fit in either category, but those are a subject for a future post! How are repressible promoters...the Q system is also functional in C. elegans. Subsequent work by the Luo and Potter labs has refined the...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Liver AAV8 Sands, 2011 Lung pUCmini-iCAP-AAV9.452sub.LUNG1 Goertsen et al., 2022 Pancreas AAV6, AAV8...designs and administration doses. Don't forget to submit your validation data after your experiment! Thanks...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...imparts TALENs more specificity and a broader substrate preference than the ZFNs.   Genetic Engineering... (1944) STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES....

  11. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...and others, we have updated our kit process substantially and have plenty of ideas for further improvements...fill and what their responsibilities are. That’s a subtle but important distinction. If I invite someone ...

  12. Important Considerations When Using AAVs

    Type
    Blog Post
    ... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...to develop “AAV 3.0”, vectors that will have substantially improved performance profiles and should be ...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal typically...burnout The suggestions in this blog add up to a substantial amount of time- most of your waking hours. This...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...to uracil without cutting DNA. Uracil is then subsequently converted to thymine through DNA replication...produces stable edits. However, this system is also subject to off-target editing based on Cas9 off-target ...

  17. New Antibody Animation!

    Type
    Blog Post
    ...different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful ...
Showing: 321 - 340 of 578 results