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  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    ...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...proteins with small molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

  5. Antibodies 101: Validation

    Type
    Blog Post
    ...Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R,...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002...

  7. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...questions concern great scientific problems, but the technology may not exist to answer the question or it might...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...

  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red and Cas9, which...for highly specific genome editing.”  Nature Biotechnology 32(6) (2014): 569-576. PubMed PMID: 24770325...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...have been making major improvements to CRISPR technology since its initial applications for genome engineering...CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol 33:755–760 . https://doi.org/10.1038/nbt.3245...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... PubMed PMID: 28585549 Kim Y, et al. Nature Biotechnology. 2017. PubMed PMID: 28191901 Listen to Our ...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...membrane, you can dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins...the bubbles in the membrane sandwich Ensure methanol concentration in transfer buffer is < 20% ...
Showing: 321 - 340 of 545 results