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  1. Antibody Plasmid Collection

    Type
    Collection
    ...R-mAbs is the NeuroMab collection - monoclonal antibodies extensively validated for neuroscience research ...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...

  2. Optogenetics Guide

    Type
    Guide
    ...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...expressed in all GABAergic neurons. In this case, the subpopulation of GABAergic neurons being activated by the...engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular processes. The...channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). When exposed to light of the correct...cations flow into the cell (yellow dots), and the neuron is activated. Optogenetics tools can be broadly...and nervous system to better map and understand neuronal circuits. Optogenetic tools have also been used...Drosophila. These tools have been instrumental in neurological disorder research, helping scientists to better...

  3. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459 ). After you...

  4. Depositor Collections

    Type
    Collection
    ...Special Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection...Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett ...

  5. AAV Viral Preps

    Type
    Collection
    ...Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1,...

  6. All Antibodies

    Type
    Collection
    ...Myc, GFP, and more. Neuroscience : Antibodies targeting proteins for neuroscience research. Institute ...

  7. Plasmid Collections

    Type
    Collection
    ...MJFF) Plasmid Collection Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Rett...

  8. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...repression system can be found in this article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard, D., & Van...different populations. Image and caption from Vigouroux et al., 2018, under a CC BY 4.0 license....

  9. Synthetic Biology - Overview

    Type
    Collection
    ... O'Shea Martin Parniske Antonio Richart Herbert Sauro Claudia Schmidt-Dannert Pamela Silver Lei Stanley...forum for synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio ...

  10. Microbiology Resources

    Type
    Collection
    ...External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens...

  11. Ras Pathway

    Type
    Collection
    ...homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 ...oncogene homolog Muscle RAS oncogene homolog Neuroblastoma RAS viral (v-ras) oncogene homolog RASA RASA1...

  12. CRISPR References and Information

    Type
    Collection
    ...construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection marker); pCFJ104 (body wall muscle... 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics ...

  14. Retrograde AAV viral preps

    Type
    Collection
    ...transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this...

  15. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation...

  16. Validated gRNA Sequences

    Type
    Collection
    ... Zhang Neurog2 H. sapiens GTCTCTATCACTGATAGGGA 64161 activate S. pyogenes 25619936 Sato Neurog2 H. sapiens...

  17. Sequencing Primers

    Type
    Guide
    ... as pBAD-R, reverse primer Puro-F GCAACCTCCCCTTCTACGAGC 3' end of puromycin resistance gene, forward primer...

  20. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...requires a unique enhancer and either Ascl1 or Neurog2 activity . Development, 148(12). doi: 10.1242/dev...
Showing: 301 - 320 of 336 results