...AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, ...

...on the Addgene Blog Origin of Replication The Promoter Region – Let's Go! Browse All of Our Plasmids ...

...amino acid protein  12 Stability At N-terminus, promotes folding and structural integrity; cleavable. Not...

...others understand ours. Improving access to data promotes dissemination, especially for young researchers...

...anabolic to catabolic processes. Active mTORC1 promotes protein and lipid synthesis, and increases energy... cancer. Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial...mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions needed for uncontrolled cell growth... which appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway ...

...plasmid (Loqué et al., 2007) containing the PMA1 promoter and the URA3 marker, the p415 plasmid (Loqué et... et al., 2007) containing the PMA1 promoter and the LEU2 marker, or the pCEV-G2-Km plasmid (Vickers et...et al., 2013) containing the TEF1 or PGK1 promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable...

...Cas enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence...Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...activator (such as dCas9-VP64) gRNA(s) targeting promoter elements of target gene Many different activators...dCas9-repressors, gRNAs should be targeted to the promoter driving expression of your gene of interest. For...

...toxicity. AAV vectors can be equipped with strong promoters for high and neuron-specific transgene expression...AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our retrograde AAV blog...

...shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression...11795 pLL3.7 3rd Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor...silencing were added; Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor...with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned into the plasmid...coexpression. Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression of RFP...

...higher level gene networks. Examples Include: promoters and terminators repressors and activators logic...depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back to Top Do you have suggestions...

...listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription...

...listed below ( MLM3639 and JDS246 ) harbor a CMV promoter that drives expression of a transcript encoding...vectors express a customized guide RNA from a U6 promoter. Individual plasmids can be ordered via the links...

...Community (MCC) Collection Pleiades Promoter Project: mini-promoters to drive selective expression in the...

...beneficial for many systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed...

...localization sequence) and expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre...

...interest. A low primer-to-template ratio is used to promote annealing of one primer to each template. The PCR...

...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...

...iteron-containing plasmids,  RepA can also bind to its own promoter and inhibit its own transcription. As copy number...

...encoded on the envelope plasmid which contains a promoter, the envelope gene, and a polyA tail.   ...

...from a replicating adenovirus using native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016...