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Showing: 301 - 320 of 590 results

  2. What the HEK?

    Type
    Blog Post
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...

  4. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools by increasing gRNA stability. First, they began with the Broccoli aptamer system to visualize the... will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In...

  6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict....2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal...

  9. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation iGluSnFR...C-terminal domain of Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for...

  10. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...

  14. How to Lead a Great Meeting

    Type
    Blog Post
    ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When...

  15. Components of CRISPR/Cas9

    Type
    Blog Post
    ... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...EQR variant 3' NGAG SpCas9 VQR variant  3' NGAN or NGNG  xCas9 3' NG, GAA, or GAT SpCas9-NG ...

  16. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ...

  18. Science Career Options

    Type
    Blog Post
    ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...

  19. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...

  20. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...
Showing: 301 - 320 of 590 results