Skip to main content
Addgene
Showing: 301 - 320 of 545 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...discovery through the creation of innovative technologies. To date his group has develop several popular...genome editing and transcription modulation. Nat Biotechnol 33:139–142 . https://doi.org/10.1038/nbt.3149...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    ...tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ... heterologous protein expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech...

  7. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  8. Lab to Office Culture Shock

    Type
    Blog Post
    ... my work. The meetings often are the work. Will Arnold, one of the newer members of our Scientist team...and for modeling in the picture above), and Will Arnold for their ideas and tips! Additional resources ...

  9. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...necessary), there are opportunities to work for biotechnology companies and help other researchers use the...experience and scientific knowledge they require. Biotechnology companies vary to what extent they hire experts...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  15. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner...
Showing: 301 - 320 of 545 results