...genetically encoded calcium indicators for targeted neuronal imaging." Nature methods 12.9 (2015): 852-858....

...University of Colorado Anschutz Medical Campus in Aurora, CO where she studies the development and regeneration...

...parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial 3D volumes. In addition...

...of the ability of a given lncRNA to cis-repress Mauro Calabrese 106253 pLS-mP-Luc Firefly Insertion of...ratiometric imaging David Nelson 212935 pGL4.84(hRlucCP/Puro) RapidResponse(TM) Renilla Vector for investigating...transcription Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for investigating regions controlling...firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly...firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of renilla luciferase...luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly...

...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...production of transgenic zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct...resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - A repository...) for the distribution of zebrafish lines from European researchers. International Zebrafish Society (...

... shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1 Lentiviral...cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root...from pCW57.1. rtTA was replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please... interest. tTA Off Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression....tTA Adenoviral; CMV promoter; see article for neuronal-specific promoter and enhancer options tTA Off...

...Ayoubi, R., Southern, K., Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium...

...specific neurons, often utilizing viral expression systems, to activate or deactivate these neurons in a .... When using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent... table below for the optogenetics plasmids for neuronal excitation and inhibition. For example, search...

..., Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators ...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV...

...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https...

...Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222...pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm...

...3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9/pMXs-puro Expresses...recognizing a telomere repeat 63590 3xFN-Tel-TAL/pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...

...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL...pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen Lab SapTrap...

...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with...

...semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016...

...receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. For more detailed descriptions of chemogenetic...

..... Gateway Cloning Brain Initiative Collection NeuroMab Collection Plasmids Pooled Libraries Feedback ...the Innovative Medicine Initiative (IMI) of the European Union. The RESOLUTE mission is to develop data...This Joint Undertaking receives support from the European Union's Horizon 2020 research and innovation programme...

...vector. 48226 pAC94-pmax-dCas9VP160-2A-puro : dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor...

...Filaments (neuronal) Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru...

...control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD Receptor... and outcome in neurons. Table 2. Descriptions of PSAMs and their activity in neurons PSAM Ion Pore Domain...Outcome (in neurons) Reference PSAM4 Gly Varenicline, uPSEM 792, uPSEM 817 Cl - influx Neuronal inhibition...influx Neuronal activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal inhibition...influx Neuronal activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal inhibition...and their activity in neurons LMO Luciferase Opsin Ligand Effect Outcome (in neurons) Reference LMO3 sbGLuc...expressed. However, neuronal control through PSAMs and LMOs is direct, while neuronal control of DREADDs...