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Showing: 281 - 300 of 520 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...receptor for SARS-CoV-2, in a single round of viral entry and infection. This approach allows researchers ...Lentiviral prep . ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...

  2. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or... recode) ID Plasmid Description Mutations Tags Industry Return to top Other Resources Addgene COVID-19...

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi...Mammalian BbsI yes, activate S. pyogenes Jaenisch piCRg Entry 58904 Mammalian BbsI yes, cut S. pyogenes Huangfu...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PMID:31170424 Poulin...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PMID:30755721 Mandelbaum G, ...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...will introduce unwanted variation into your data. Try different types of pipettes and pipetting methods...application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries are hydrolysis...primer/probe actions to a single step. Scorpion chemistry in particular has been reported to work well in...features of several commonly used chemistries)   Chemistry Background noise Probe Allelic specificity...to need to adjust amounts of reagents in your chemistry, unless running a multiplex reaction or on a rapid...cycle machine. Melt Curves An advantage of Sybr chemistry is the ability to run a melt curve to validate...multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...activation domain is an incredible tool for researchers trying to activate target genes, the relatively large ...to customize for targeting your gene of interest. Try them today! Ordon J, et al. Plant J. 2016. PubMed...food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend on the yeast... pull-down methods and identified using mass spectrometry. The Roux Lab’s new and improved version of ...were validated by both Western blotting and immunohistochemistry, and are summarized in the table below: ...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...No. 5, November 2011, pp. 313–327 Scholarpedia : Entry by Robert Campbell Spectra Database : Graphing site...2012, Vol. 134 No.18, pp. 7913–7923 Hoi et al. : Chemistry & Biology, October 2013, Vol. 20 No. 10, pp.1296...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...other inntags available through Addgene so you can try these incredible new tools yourself! The tags can...their wild-type counterparts - a real boon to those trying to understand physiological interactions and functions...set of 12 promoter-less Gateway® entry clones to be used with other entry clones encoding the ORFs of interest... article through Addgene. Nihongaki et al., Chemistry and Biology. 2015 Feb 19; 22(2):169-74. High...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...traditional restriction enzyme cloning to create an Entry vector containing a promoter for constitutive (CMV...expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato...

  11. Browse

    Type
    Collection
    ...Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

  17. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium ...

  18. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...

  20. Synthetic Biology - Overview

    Type
    Collection
    ...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository of community-created...
Showing: 281 - 300 of 520 results