Quick Guide to All Things Lentivirus
Type
Blog Post
...particles and the virus particles produced could not replicate. However, Mother Nature found a way to re-create...packaging plasmid forming a vector that could create replication competent virus. Even though this system was ...still a potential risk for the generation of replication competent lentiviruses (RCL) especially if you...factors in HIV positive cells could make the virus replication competent.
The 3rd generation of lentiviral...transgenes (>8 kb)
Potential for generation of replication-competent lentivirus (RCL)
Efficient gene transfer...transfer
Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human tropism can... incubate your cells with virus at different multiplicity of infection (MOI=ratio of virus to cells). ...