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We narrowed to 532 results for: invs

Showing: 281 - 300 of 532 results

  1. March for Science

    Type
    Blog Post
    ...march is meant to inspire scientists to be more involved in their communities (something already encouraged...mathematical constant “pi” supports scientists getting involved in politics. Disclaimer: The views presented ...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... a suicide switch in cyanobacteria, synthetic inverters in E. coli, and biosensors in E. coli: Čelešnik...017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs." PloS...

  3. Evolution of Lab Techniques

    Type
    Blog Post
    ...real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be used to detect ...commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing the fragments...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Traditionally, delivery is achieved through the use of invasive methods, limiting researchers to accessible, spatially...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... Gradinaru, V. (2022). Engineered AAVs for non-invasive gene delivery to rodent and non-human primate ...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...to perform functional screens to identify genes involved in a phenotype of interest. This blog post will... is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent...

  6. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...How are their organizational skills? Are they involved with colleagues and the department? Are they ...transferable skills by attending career events or being involved in a grad or postdoc association? Will you learn...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... fragment library of phage DNA This first step involves digesting phage genomic DNA into 1-3 kb (approximately...fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening using compressed...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    ...working in a rural health clinic in Ghana. My role involved teaching the nursing students and community health... future supervisors were keen for me to become involved with many different aspects of the company and...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...Network blog for more ideas of when and how to get involved.  Why do you do science policy? I would argue...Journal of Science Policy and Governance.  Get involved! The best way to learn about science policy and...

  12. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...That’s a subtle but important distinction. If I invite someone to be on a project team, I try to make ...next team works better. In fact, don’t wait to be invited onto a team. Make one yourself. Instead of thinking...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...

  14. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ... IV-B have led to the hypotheses that they are involved in plasmid competition or can hijack machinery...Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

  17. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...themselves, use the blood stream to commute to and invade multiple organs, and most importantly escape the...tumor cells and immune cells to knockout proteins involved in immune tolerance. Recently, China spearheaded...

  18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...structurally characterizing large ion channels involved in skeletal muscle contraction.   References 1...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...’s be honest — kits are quick, easy, and don’t involve phenol-chloroform.  The default option is to use...approaches — columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate...
Showing: 281 - 300 of 532 results