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  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient and less toxic to...

  3. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...experiencing sustained and substantial relief from both disorders. As of publication, submissions to the UK, EU, ...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...-wrmScarlet labeling proteins with different subcellular locations. Image from Goudeau et al., 2020. ...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...want to consider using an aliquot that has been subjected to a freeze/thaw cycle for titering as this will...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...Essentially this reporter construct consists of one substrate (the histone-derived peptide) which can localise...together and causes an increase in the FRET signal. Subsequent demethylation separates the fluorescent units...

  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... using reverse transcription and the cDNA is subsequently quantified by qPCR. qPCR is used in a variety...Variations Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex PCR...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...providing enzyme protection while still allowing substrates and products to diffuse freely into or out of...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate transcription...propagation, Hu et al. isolated xCas9 1.0-1.4 and subjected them to another 72 hours of phage-assisted evolution...

  14. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...presented with a physical model of his large ribosomal subunit, he kept sliding his palm across the surface of...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...

  16. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... contest for about two months, and we got one submission. I was a bit disappointed in the lack of participation...on what the experience taught me. The article submitted was hilarious, full of interesting information...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...this reconstruction lay the foundation for all subsequent versions of LSFM microscopes (3,4). After this...relied on the use of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e....

  18. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...checkbox and paste your reference sequence into the Subject Sequence box that appears. Aligning two nucleotide...pasting your expected protein sequence into the Subject Sequence box. BLAST alternatives Depending on your...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues in ... on the ADAR2DD residues that contact the RNA substrate resulted in 15% C-to-U editing activity. They ...
Showing: 281 - 300 of 576 results