...library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and...library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ...

...Research Foundation Neuroendocrine Tumor Research Foundation The mission of the Neuroendocrine Tumor Research...funding from the Neuroendocrine Tumor Research Foundation Depositor... Depositor Collections Neuroendocrine Tumor Research...treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and... progress in the diagnosis and treatment of neuroendocrine cancers. As such, NETRF strongly encourages...Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids...Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-Farber Cancer...

...targeted neuronal manipulation. Topics may include: How this technology is used for targeted neuronal manipulation...Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September 21, 2018 Contact... AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional control). The...fit in the big picture of methods for targeted neuronal manipulation? Best practices for using this techonology... is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables, data, or images...communication representing a reference for AAV use in neuronal intersectional control. Through your participation...Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Haery L, Deverman BE, Matho KS, ...

...expression Tyler Jacks 11586 pSico PGK puro Cre addition causes the puromycin gene to be recombined out of the...expression Tyler Jacks 12084 pSicoR PGK Puro Cre addition causes both puromycin and shRNA to be recombined out ...PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative...pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative of pMKO.1 puro with GFP... GFP instead of puromycin resistance gene William Hahn Additional Resources Web References Read our detailed...

...with an in vitro transposition reaction." BMC neuroscience 3.1 (2002): 7. PubMed PMID: 12086589. PubMed...

...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...

...have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016). For these...

...Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503...

...semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016...

...for labeling these cells. Check out Addgene’s Neuroscience Antibody Collection to find a recombinant anti-GFAP...

...potential tools for eradicating HIV-1/AIDS. J Neurovirol. 2015 Jun;21(3):310-21. PubMed PMID: 25716921...

...23663447. PubMed Central PMCID: PMC3684516. 5. Casini, Arturo, et al. "One-pot DNA construction for synthetic...

...antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. 2014 Feb;24(1):...

...Philosophy at Harvard University) Tony Cijsouw (Neuroscience postdoc at Tufts University) Scientific reproducibility...

...expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral ...plasmids for drosophila expression pMT-puro - Inducible gene expression under...destination vector for gene expression Puromycin Mammalian pLKO.1 - TRC cloning ...Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination...Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA ... expression, multiple selectable markers pMKO.1 puro - Mammalian retroviral shRNA expression...for mammalian gene silencing pMKO.1 puro - Retroviral plasmid for mammalian...

... expression; also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO...CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-... for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid; deletion...

...Torensma R, Turley H, Pulford K, Banham AH (2015) The European antibody network’s practical guide to finding ...

...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...

...PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1...

...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...