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Showing: 261 - 280 of 509 results

  1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...spread from the injection site it is advisable to try a few different injection titers and volumes to determine...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https:/...

  2. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...or if no monomers exist in your sample, you could try linearizing the plasmid via restriction digestion...colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

  3. Cre-lox system

    Type
    Collection
    ...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian...with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko 139547...Cre T. brucei Cross 33345 pLEW100cre-EP1 Cre Trypanosoma brucei Cross 34564 pMSCVpuro-Cre Cre 5'LTR Retroviral...components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish Eisenhoffer 131783 pTol2...139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin Plant...

  4. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing...email updates for the newest plasmids available to industry. These plasmids have not been expressed or functionally... recode) ID Plasmid Description Mutations Tags Industry Return to top Other Resources Addgene COVID-19...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Jul 29;7:12266. David Savage Tryptophan Visualize intracellular tryptophan levels and flux Nanosensor detection...green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green- and...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity. PLoS One. 2015 Apr 14... Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. Martin Webb Phosphate... detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10)... Grx1-roCherry. Redox Biol. 2019 Feb;21:101071. Dmitry Bilan Temperature Genetically encoded ratiometric...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...kinase-library reactions (SERIOHL-KILR, Barber et al, Biochemistry 2018). Iterative Synthetically Phosphorylated..., Jun JW, Lou HJ, Turk BE, Rinehart J. 2018. Biochemistry . 2018 Aug 7;57(31):4717-4725. PubMed Improved...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...its substrate. Then, all it takes is a bit of chemistry magic to produce a wide range of useful molecules...incorporate an alkene to be labeled through click chemistry. Although these multi-component systems can be...brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and HaloTag ligands...Tag for Multiprotein Labeling in Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016...L. (2016, September 8). Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https...https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell...

  9. Antibody Production

    Type
    Collection
    ...Purity Antibodies are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed...your lot, please email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the...is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized...

  10. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG, Myc, V5, or HA). ...pull-down assays include immunoprecipitation mass spectrometry (IP/MS) and chromatin immunoprecipitation (ChIP... protein complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly...analyzed for tagging efficiency using standard immunocytochemistry (ICC) assays.  Figure 1: Three ingredients...single-stranded donor DNA (V5 tag) were quantified by immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069...

  11. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...full list below. ID Plasmid Gene/Insert Mutations Industry Return to Top MJFF Shared Research Tools Labs ...list below. ID Plasmid Description Gene/Insert Industry PI Return to Top...

  12. All Antibodies

    Type
    Collection
    ...currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue to work...

  13. Resolute Plasmid Collection

    Type
    Collection
    ... public-private partnership of 13 members from industry and academia financed by the Innovative Medicine...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HTR3 HTR3B 5-hydroxytryptamine (serotonin) receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor... HTN3 histatin 3 HIS2, HTN2, HTN5 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-... receptor 3, family member C - HTR3D 5-hydroxytryptamine (serotonin) receptor 3 family member D MGC119636...MGC119636, MGC119637 HTR3E 5-hydroxytryptamine (serotonin) receptor 3, family member E 5-HT3c1, MGC120035,...factor receptor superfamily, member 14 (herpesvirus entry mediator) ATAR, HVEA, HVEM, LIGHTR, TR2 TNFRSF17...Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David ...

  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...receptor for SARS-CoV-2, in a single round of viral entry and infection. This approach allows researchers ...Lentiviral prep . ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi...Mammalian BbsI yes, activate S. pyogenes Jaenisch piCRg Entry 58904 Mammalian BbsI yes, cut S. pyogenes Huangfu...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    ...accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PMID:31170424 Poulin...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PMID:30755721 Mandelbaum G, ...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ...will introduce unwanted variation into your data. Try different types of pipettes and pipetting methods...application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries are hydrolysis...primer/probe actions to a single step. Scorpion chemistry in particular has been reported to work well in...features of several commonly used chemistries)   Chemistry Background noise Probe Allelic specificity...to need to adjust amounts of reagents in your chemistry, unless running a multiplex reaction or on a rapid...cycle machine. Melt Curves An advantage of Sybr chemistry is the ability to run a melt curve to validate...multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in...
Showing: 261 - 280 of 509 results