...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette tips, 20 µL, VWR 76322-134 Pipette tips, 200 µL...VWR 76322-150 Pipette tips, 1000 µL, VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 Pipettes, 10 mL, VWR 89130...single channel pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking ... 89130-898 Pipettes, 25 mL, VWR 89130-900 Pipettes, 50 mL, VWR 89130-902 Gel loading tips, Corning 4853...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous...Neutralize trypsin by adding 10 mL of DMEM Complete. Pipet back and forth vigorously multiple times to obtain...with the cells harvested in the previous step. Pipette back and forth or shake vigorously to obtain a ...total of 5 mL of cell lysis buffer (recipe above). Pipet back and forth to resuspend each pellet completely...adding a total of 12.5 mL of cell lysis buffer. Pipet back and forth to resuspend each pellet completely...

...spread antibiotic over-agar. Equipment Pipette tips for both pipetting and spreading Bunsen burner (or other...expensive. With a 6 cm diameter petri plate containing solidified LB-agar, pipette 150 µL of carbenicillin on...liquid remaining on the surface). We use the micropipette tip itself to do the spreading; the tip is gently...used for this purpose, provided that they fit your petri plate. Incubate the plate at room temperature for...same way as the antibiotic, using either a bent micropipette tip or other cell spreading device that fits...

...Fix and label cells Equipment Pipette controller Pipette tips and pipettes Rocking platform Tweezers Fluorescent...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Reagents 1X PBS Microcentrifuge tubes Sterile Poly-D-lysine coated coverslips HeLa...

...single channel pipette Pipette controller Pipette tips and pipettes Spectrophotometer Heat block Mini gel...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...

...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression...M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel extraction...hours. Transform 2 μL of ligation mix into 25 μL competent DH5 alpha cells, following manufacturer’s protocol...OPTI-MEM In a polypropylene tube, add OPTI-MEM first. Pipette FuGENE® directly into the OPTI-MEM – do not allow...tube from step c for a total volume of 100 μL. Pipette master mix directly into the liquid and not onto...cells. Swirl to disperse mixture evenly. Do not pipette or swirl too vigorously, as you do not want to ...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted fluorescence...cells into 14 mL of DMEM complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension ...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir...out of the previously seeded 10 cm plate. Slowly pipette the transfection mix onto the 10 cm plate, being...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM...complete containing 10 μg/mL polybrene. Mix well by pipetting or inverting. Aliquot 1.35 mL of the cell suspension...

...Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes Benchtop centrifuge compatible...Reagent Preparation instructions. Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent...Check the cell density and viability: Using a 5 mL pipette, transfer 0.5 mL of HEK293 cell suspension into...the microfuge tube containing the trypan blue. Pipette 10 times to mix. Load 10 µL of the cell suspension...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM...DMEM complete + 10 µg/mL polybrene. Mix well by pipetting or inverting the tube. Aliquot 1 mL of cell suspension...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope Hemocytometer... by passing several times through a serological pipet or by passing through a 40 µm cell strainer mesh...

...storage in the cold room may damage it. Using a pipettor, transfer the top, aqueous phase to a new RNAse-free...disturb or collect the interphase layer with your pipette tip. Pro-Tip To collect as much of the top aqueous...interphase layer, consider using a lower volume pipettor like a p200 to collect a majority of the aqueous...

...single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single...single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM...

...is unloaded, push the pipettor to the second stop and carefully raise the pipette straight out of the buffer...comb or towards the sides/edges of the gel with a pipette tip. Place newly poured gel at 4 °C for 10-15 mins... the tip. Place the very top of the tip of the pipette into the buffer just above the well. Very slowly...needed because several microliters can be lost in pipetting. For example, if you want to load 1.0 μg in 10...

...want to use electro-competent cells instead of the more common chemically-competent cells. The number of...Follow the manufacturer’s instructions for your competent cells. For most standard cloning, you can transform...transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much...getting colonies, you might want to use higher competency cells. Additionally, if your final product is...

...want to use electro-competent cells instead of the more common chemically-competent cells. The number of...according to the manufacturer’s instructions for your competent cells. For most standard cloning, you can transform...transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much ...getting colonies, you might want to use higher competency cells. Additionally, if your final product is...

...Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and...and avoid inconsistent pipetting with our simple tips Bacterial Transformations We walk you through a standard...

...use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, how to dispense...dispense liquids, and how to handle the pipette Watch the Video! Centrifugation Learn about selecting and ...

...digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of...instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction enzymes to digest....2-0.5 µL of enzyme because it is difficult to pipette less volume than this; 0.2-0.5 µL will likely be... more enzyme is usually better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually...