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TypeBlog Post...e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure their...elegans gRNA sequences. You can also filter by multiple keywords that are separated by a single space ...
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Easing the Protein Purification Process with pCri
TypeBlog Post...protein purification through simple and efficient multiplexing. pCri plasmids are divided into two sets, each...schematic below), enabling a researcher to test multiple tags for one protein quickly and easily. These... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...10;4:2153. doi: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...Development-specific genes – if a gene functions at multiple stages in development, its depletion may result...be observed. Functionally redundant genes – multiple genes which function in overlapping roles can ... -
Fostering communication in remote collaborations
TypeBlog Post...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss... -
Plasmids 101: What is a plasmid?
TypeBlog Post...for selection of plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which ...maintain your plasmid stocks. The guide also contains multiple protocols and troubleshooting tips to make plasmid... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted in.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so...HDR mentioned above, an accessible germline, and multiple selectable markers. Bob Goldstein’s lab previously... -
Another Pathway into Cells: iTOP
TypeBlog Post...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...word “extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...to create an array of multicolor reporters that will enable imaging of multiple proteases at once (Zhang... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post... therapy, however, because the disease affects multiple organ systems. Nevertheless, the findings are ... Multiple Authors... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes ... -
Viral Vectors 101: Biosensors
TypeBlog Post... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in... -
Viral Vectors 101: Types of viruses
TypeBlog Post...administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more synthetic...enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus, or ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...therapies have already been approved in the US for multiple diseases, including hemophilia B and spinal muscular... Multiple Authors... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...molecular biology and scientific research. There are multiple ways foreign DNA can be introduced into cells ...References Baltrus, David A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter...