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  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair from...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...1993). But why did this technique fail to gain traction? It is likely that both vector amplification with...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... and regulations impacting grant management, we track grant opportunity deadlines, and we ensure that ...questions when you’re negotiating a new job or a new contract. Connect with other grant professionals on LinkedIn...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... the transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...was successful at all. Once we were on the right track, we switched to luciferases to more easily quantify...

  13. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...with AAV sgRNA delivery make the SPH mouse an attractive tool to probe gene-function relationships in ...cell populations makes Cre-dependent SPH mice an attractive alternative. Whatever your application, first...

  14. Viral Production at Addgene

    Type
    Blog Post
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...vector, incubated for 72 hours, and genomic DNA is extracted. Primers and probes targeting integrated copies...

  16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation promotes...

  19. Five Popular Model Organisms

    Type
    Blog Post
    ... model organisms is that they are genetically tractable. Mice can be easily manipulated with tools like...al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming...
Showing: 261 - 280 of 508 results