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  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...

  2. Hot Plasmids - May 2022

    Type
    Blog Post
    ... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

  4. Hot Plasmids - February 2022

    Type
    Blog Post
    ...live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting, and more....future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...

  5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...you have any questions or thoughts about this technology, leave them in the comments below.  References... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    ...References and resources References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler... profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler...

  8. To Each HIS Own

    Type
    Blog Post
    ...Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...running and more time working on interesting biotechnology code.There are valid technical reasons to choose...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....

  12. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

  16. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...

  18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...team will communicate with your organization’s technology transfer office or other authorized office directly... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

  20. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...Cre is expressed.  Several variations of this technology can be used to customize the effects of this ...
Showing: 261 - 280 of 545 results