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...Decades later and after years of painstaking work trying to isolate the causative gene for Rett Syndrome...

...position and directionality of the sequence you are trying to clone. Figure 2: MoClo assemblies depend...

...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...

... new to the mARG expression are advised to first try to produce GVs in their own lab using the most reliable...is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar...Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209 (2017). Bourdeau, R. W. et al....

...what the ploidy of your target site is. If you are trying to introduce a homozygous mutation, it’s helpful...

...this and are still struggling with idea generation, try this thought exercise: Consider the issue as if there...

... Addgene. If you’ve given any of these systems a try, we’d love to hear your feedback!   Thank you to...Jieze Zhang is an undergraduate student studying biochemistry at the University of Southern California. He...obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese Academy of Sciences. He joined Shanghai... new generation of engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered...

...question of how we can support researchers as they try to keep up with the growing amount of interdisciplinary...

...Volunteer moderators set the rules for these forums and try to keep discussions on-topic and, at least sometimes...

... know a lot about and that you care about. Don’t try to present topics that are unfamiliar to you because...Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...

...directed mutagenesis. In addition, you may want to try different concentrations of template (e.g. 0.1-1.0...in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID: 8470801....

...this and thinking “Gee, I’d love to give these a try myself,” look no further! You can package your own...virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting these three plasmids...

...have a significant impact on your data. You are trying to generate a knockout cell line, with the goal...

...phosphorylation affects protein function, researchers should try to reverse the effects of pSer-incorporation on enzyme... model systems coupled with advances in mass spectrometry suggest that phosphorylation is far more prevalent...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated... pSer incorporation can also done using mass spectrometry to identify a mass shift proportional to a single...

...synthetic biology, you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, ...

...the closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing...

...insertion site. If you are using the ivTRT method, try to make the first two bases of the left homology ...

...support, and distribution capabilities. Give HiBiT a try and see your favorite proteins in a whole new light... green fluorescent protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem...

... issue. This may be more challenging if you are trying to apply for an outright job in a field where you...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...enter this career trajectory. One common route of entry is through a science policy fellowship program which...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

...type- and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...