...Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet... quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4. Hindson CM, Chevillet...

...question of how we can support researchers as they try to keep up with the growing amount of interdisciplinary...

...Volunteer moderators set the rules for these forums and try to keep discussions on-topic and, at least sometimes...

... know a lot about and that you care about. Don’t try to present topics that are unfamiliar to you because...Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...

...directed mutagenesis. In addition, you may want to try different concentrations of template (e.g. 0.1-1.0...in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID: 8470801....

...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...

...pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid or an organism’s ... and Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ...

...the closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing...

...synthetic biology, you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, ...

...Nelson et al., and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation ...

...if you’re working with plants, don’t be afraid to try your hand at CRISPR. References Belhaj K, Chaparro-Garcia...

...phosphorylation affects protein function, researchers should try to reverse the effects of pSer-incorporation on enzyme... model systems coupled with advances in mass spectrometry suggest that phosphorylation is far more prevalent...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated... pSer incorporation can also done using mass spectrometry to identify a mass shift proportional to a single...

...type- and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...

...have a significant impact on your data. You are trying to generate a knockout cell line, with the goal...

...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common...

... issue. This may be more challenging if you are trying to apply for an outright job in a field where you...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...enter this career trajectory. One common route of entry is through a science policy fellowship program which...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing...this novel virus. It is now known that SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on ...plasmids that have been used for measuring viral entry and activity in neutralization assays and links ...growing. ID Plasmid Description Industry PI Other Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID...ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials are ... as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find plasmids related...

... Plasmid Description Industry Attachments mEmeralds Plasmid Description Industry Attachments Reds Plasmid...Plasmid Description Industry Attachments Blues Plasmid Description Industry Attachments Cyans Plasmid Description...Description Industry Attachments Oranges Plasmid Description Industry Attachments Greens Plasmid Description...Description Industry Attachments Yellows Plasmid Description Industry Attachments Photoactivatables Plasmid...Plasmid Description Industry Attachments Do you have suggestions for other plasmids that should be added ...

...or if no monomers exist in your sample, you could try linearizing the plasmid via restriction digestion...colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

...expression in a variety of cell types, but you should try to find the best AAV for your particular experiment...: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15 (2005): 9933...