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  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...of saying “I have excellent communication skills” try giving an example, like “I have extensive communication... Should I write a cover letter? If you’re in a country where cover letters are common, the answer is usually...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover letters...if ever, sees cover letters submitted for their entry-level jobs and does not require or request them ...format regardless of whether you’re in academia, industry, or nonprofits. Like any other letter, they have...positions. Many did not require cover letters for entry-level positions, as at that stage, cover letters...
  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter of time... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with...Minnesota State (and now Professor of Chemistry and Biochemistry at the University of San Diego), decided...Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth...presented their findings at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of...inquiry and research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification...dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323...
  3. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...and free for all non-profit uses. We invite you to try ICE today and let us know how it works for you! Read...the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth analysis will...
  4. Lab to Office Culture Shock

    Type
    Blog Post
    ...organizations like Science from Scientists, which try to bring real scientists into schools to provide ... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...
  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...organisms, they can be problematic for researchers trying to express proteins in heterologous hosts. If you...
  6. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...applying for technical support positions, you should try to match your expertise with that of the company....are often found in sales or lab-based roles in industry. What to look forward to in a technical support...offer an easy transition from an academic lab to industry without being too commercial or “sales-y”. The...be a good jumping off point for other roles in industry. Many thanks to Eric Perkins and Stephanie Hays...
  7. Plasmids 101: Protein tags

    Type
    Blog Post
    ...their protein of interest. After several decades of trying to address these challenges, researchers have amassed... used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding... used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires...
  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...not always a straightforward process. Rather than try to directly detect your gene of interest, scientists...advantage of an element called an Internal Ribosome Entry Site (IRES) to allow for initiation of translation...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et...
  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters...E. coli tryptophan operon Repressible Gets turned off with high levels of cellular tryptophan. lac General...
  10. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...microfuge tube and vortex. Pro-Tip You may need to try a few different concentrations of antigen to determine...101: The Four ELISAs and When to Use Them Immunocytochemistry Protocol Recombinant Antibody Purification...
  11. Molecular Biology Reference

    Type
    Guide
    ...pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid or an organism’s ... and Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ...
  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...
Showing: 221 - 240 of 520 results