Addgene

We narrowed to 292 results for: pET

Showing: 221 - 240 of 292 results

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Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...
Hot Plasmids - August 2020

Type

Blog Post

...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...Tetracycline RecA-deficient; best for plasmids with repetative sequences. F- ompT lon hsdSB (rB- mB-) gal ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...
Tips for Getting a Faculty Position

Type

Blog Post

...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

...of time and effort and give the lab a serious competitive advantage. Many thanks to our guest blogger...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...
Generating Mouse Models Using CRISPR/Cas9

Type

Blog Post

...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker...
PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4...
Finding Your Perfect Job After University

Type

Blog Post

...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic...
A History of Genome Engineering in Popular Culture

Type

Blog Post

...successful in the box office, indicating the insatiable appetite of the moviegoers for dinosaurs wreaking havoc...
Finding Your Science Policy Path

Type

Blog Post

...system? Defining your why is what will make you a competitive candidate for any science policy position and...
With an Eye Towards the Future, We Look Back at the March for Science

Type

Blog Post

... However, many scientists operate in a hyper-competitive environment where getting hired hinges on performing...

Showing: 221 - 240 of 292 results

Results per page:

Plasmids 101: Stringent Regulation of Replication

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Hot Plasmids - August 2020

Getting the Most from Your Lentiviral Transduction

Plasmids 101: E. coli Strains for Protein Expression

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Tips for Getting a Faculty Position

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Using Phosphoserine to Study Protein Phosphorylation

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Generating Mouse Models Using CRISPR/Cas9

PiggyBac-ing Through the Genome Editing Field

Finding Your Perfect Job After University

Plasmids 101: Using Transposons in the Lab

A History of Genome Engineering in Popular Culture

Finding Your Science Policy Path

With an Eye Towards the Future, We Look Back at the March for Science

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