We narrowed to 304 results for: pET

1. Plasmids 101: Codon usage bias

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   Blog Post

   ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...

2. Transferable Skills Guide: External Collaborations

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   Blog Post

   ...But, as so often happens, one gets better with repetition. I learned to look at the event from the company...

3. Designing Your Chalk Talk for the Academic Job Interview

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   ...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive...

4. Lab to Office Culture Shock

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   ...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...

5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

6. Hot Plasmids - August 2020

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   ...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...

7. Transferable Skills Guide: Creativity

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   Blog Post

   ...chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have to have...

8. Antibodies 101: Immunoprecipitation

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   Blog Post

   ...serum immunoglobulins indiscriminately, causing competition for binding sites. In cases like these, antibodies...

9. Plasmids 101: Stringent Regulation of Replication

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   ...number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA...

10. Plasmids 101: Gateway Cloning

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    ... are performed, the next step is to transform competent E. coli cells and select the positive clones. ...

11. AAV Titers: Where Do They Come From And What Do They Mean?

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    ...linear region of the assay. This may be due to competition for binding sites between the primers and the...

12. Viral Vectors 101: Pseudotyping

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    ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

13. CRISPR-mediated Plant Base Editors

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    ...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...

14. Getting the Most from Your Lentiviral Transduction

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    Blog Post

    ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...

15. Plasmids 101: E. coli Strains for Protein Expression

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    ...Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal ...

16. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...of time and effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger...

17. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post

    ... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...

18. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    ...the production of lentiviral vectors that are competent for one round of infection (AKA transduction in...

20. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...