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We narrowed to 292 results for: pET

Showing: 221 - 240 of 292 results

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AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

...linear region of the assay. This may be due to competition for binding sites between the primers and the...
Transferable Skills Guide: Creativity

Type

Blog Post

...chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have to have...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...
Hot Plasmids - August 2020

Type

Blog Post

...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...Tetracycline RecA-deficient; best for plasmids with repetative sequences. F- ompT lon hsdSB (rB- mB-) gal ...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

...of time and effort and give the lab a serious competitive advantage. Many thanks to our guest blogger...
Tips for Getting a Faculty Position

Type

Blog Post

...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs...
Finding Your Perfect Job After University

Type

Blog Post

...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research...
Generating Mouse Models Using CRISPR/Cas9

Type

Blog Post

...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker...
PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4...
A History of Genome Engineering in Popular Culture

Type

Blog Post

...successful in the box office, indicating the insatiable appetite of the moviegoers for dinosaurs wreaking havoc...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic...
Finding Your Science Policy Path

Type

Blog Post

...system? Defining your why is what will make you a competitive candidate for any science policy position and...

Showing: 221 - 240 of 292 results

Results per page:

AAV Titers: Where Do They Come From And What Do They Mean?

Transferable Skills Guide: Creativity

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Hot Plasmids - August 2020

Designing Your Chalk Talk for the Academic Job Interview

Plasmids 101: E. coli Strains for Protein Expression

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Tips for Getting a Faculty Position

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Using Phosphoserine to Study Protein Phosphorylation

Finding Your Perfect Job After University

Generating Mouse Models Using CRISPR/Cas9

PiggyBac-ing Through the Genome Editing Field

A History of Genome Engineering in Popular Culture

Plasmids 101: Using Transposons in the Lab

Finding Your Science Policy Path

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