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  1. Antibodies 101: ChIP

    Type
    Blog Post
    ...    Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay...actually celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even...

  2. All in a Twist: dsRNA

    Type
    Blog Post
    ... molecules are clearly associated with cellular damage and dysfunction, it is unclear what the precise... that is now vulnerable to various forms of DNA damage, and these structures can in turn lead to genome...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... space that would size-exclude an antibody. In imaging applications, smaller affinity reagents means higher...often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two ...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ...

  5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 ...desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682/tutorials...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep 20;111(6):1119-31...2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically encoded calcium...

  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...which will bind and light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting... avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag could pay...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...

  14. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...

  15. Transferable Skills: Negotiation

    Type
    Blog Post
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...   Figure 2: Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license...   Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...fluorescent label, which can reduce the potential image resolution of super-resolution fluorescence microscopy...Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex...
Showing: 221 - 240 of 454 results