...are better for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred...involve enhancing optical live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc...mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during...

...couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition...will combine all of these. Addgenies have been organizing Halloween festivities for years – searching our...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...

...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...how to manage them without them paralyzing an organization. I think these are things that I can bring to...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...

...example, entering ‘C. elegans’, or ‘elegans’ will filter the table to show C. elegans gRNA sequences. You...

... are unclear. Dr. Di Schiavi plans on using C. elegans as a model to identify SMN1 interacting partners... of smn1 disruption. As part of his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize...genes, CRISPR to disrupt their function, and C. elegans overexpression plasmids to upregulate their expression...

...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...

...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...induces changes in a fluorescent signal based on ligand presence or changing cellular conditions. Other...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...

...specifically manipulate a cell type of interest in an organism, all cells of that type are affected. Previous...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...

... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...responsive to members of the ubiquitous activin ligand family, including Activin A. A commercially developed...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...

...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...

...process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy...provides limited information about what materials or organelles are being targeted for autophagy. The development...for reporting vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed...

...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...

...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville...

...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...

... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...

... at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission is to strengthen...respective fields. To help attendees launch new organizations that will extend opportunities to practice science... create them.  Seek out collaborations with organizations that practice science communication or start...

...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created a flexible system...Given that the liver is an immunologically active organ, this advance is likely to afford the ability to...

... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....

...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...